Hedgehog信号通路中Shh、Ptch在维吾尔族妇女宫颈癌中的表达及其关系

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摘要目的探讨Shh、Ptch蛋白在新疆维吾尔族妇女宫颈癌中的表达及与盆腔淋巴结转移的关系。方法采用实时荧光定量及免疫组织化学检测32例正常宫颈组织、43例中重度宫颈上皮内瘤变组织(CINⅡ～Ⅲ)和80例宫颈癌组织中Shh、Ptch蛋白的表达情况,分析两者在宫颈癌组织中表达的相关性及与宫颈癌盆腔淋巴结转移的关系。结果 Shh、Ptch蛋白在宫颈癌组织的阳性表达率分别为79.0％和69.7％,在CINⅡ～Ⅲ组织中的阳性表达率分别为82.5％和80.0％,均显著高于正常宫颈组织(0,12.5％),差异有统计学意义。宫颈癌中Shh蛋白的表达与Ptch蛋白的表达呈正相关(r=0.672,P＜0.05)。将宫颈癌盆腔淋巴结转移组与无转移组进行单因素比较分析,Shh蛋白表达、Ptch蛋白表达、组织学分化程度、脉管浸润、肌层浸润深度与宫颈癌盆腔淋巴结转移有关,进一步Logistic回归分析表明,Shh蛋白表达、肌层浸润深度是宫颈癌盆腔淋巴结转移的独立危险因素。结论 Shh、Ptch蛋白异常表达可能协同参与宫颈癌的恶性进展,Shh蛋白高表达是宫颈癌盆腔淋巴结转移的独立危险因素。

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	朱明玥
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	古扎丽努尔·阿不力孜
	唐努尔

关键词 ：宫颈癌, Shh蛋白, Ptch蛋白, 盆腔淋巴结转移

Abstract：Objective To quantify the expression of Shh and Ptch proteins in cervical carcinoma and to examine their role in metastasis. Methods The expression of Shh and Ptch in 32 specimens of normal cervical tissue, 43 specimens of moderate-severe cervical intraepithelial neoplasia, and 80 specimens of cervical carcinoma was measured by real-time quantitative PCR and immunohistochemistry. The correlation between Shh and Ptch proteins in cervical carcinoma was analyzed; the correlation of the 2 proteins with pelvic lymph node metastasis was also evaluated. Results The positive expression rates in cervical carcinoma and CIN II-III were 79.0% and 82.5% for Shh, and 69.7% and 80.0% for Ptch, respectively. These rates were significantly higher than those of the normal cervical tissue. Shh expression was positively correlated to Ptch expression (r=0.672, P<0.05). The single factor comparative analysis between metastasis group and none-metastasis group showed that the expression of Shh and Ptch, histological differentiation, vascular invasion, and muscle invasion depth were associated with cervical carcinoma pelvic lymph node metastasis. Logistic regression analysis showed that Shh expression and muscle invasion depth were independent risk factors for cervical carcinoma pelvic lymph node metastasis. Conclusion Deregulation of Shh and Ptch may synergistically participate in the malignant progression of cervical carcinoma. The expression of Shh is an independent risk factor for cervical carcinoma pelvic lymph node metastasis.

Key words： Cervical carcinoma Shh Ptch Pelvic lymph node metastasis

收稿日期: 2015-01-26

通讯作者: 古扎丽努尔·阿不力孜(1269628592@qq.com)

引用本文:

朱明玥, 陈帆, 李华, 古扎丽努尔·阿不力孜, 唐努尔. Hedgehog信号通路中Shh、Ptch在维吾尔族妇女宫颈癌中的表达及其关系[J]. 中国生育健康杂志, 2015, 26(6): 518-524.
ZHU Mingyue, CHEN Fan, LI Hua, GUZHALINUER·Abulizi, Tangnuer. Expression of Shh and Ptch proteins in cervical carcinoma and their correlations with pelvic lymph node metastasis. Chinese Journal of Reproductive Health, 2015, 26(6): 518-524.

链接本文:

http://cjrh.bjmu.edu.cn/CN/ 或 http://cjrh.bjmu.edu.cn/CN/Y2015/V26/I6/518

1 Nussle-Volhard C,Wieschaus E.Mutations affecting segment number and polarity in Drosophila.Nature,1980,287:795-801.
2 Cohen MM Jr.The hedgehog signalling network.Am J Med Genet A,2003,123A:5-28.
3 Gupta S,Takebe N,Lorusso P,Targeting the Hedhehog pathway in cancer,Ther Adv Med Oncol,2010,2:237-250.
4 Theunissen JW,de Sauvage FJ.Paracrine Hedgehog signaling in cancer.Cancer Res,2009,69:6007-6010.
5 曹泽义,主编.中华妇产科学.第2版,北京:人民卫生出版社,2004,11:2010-1061.
6 张克松,李克.Hedgehog 信号途径与肿瘤.中华肿瘤防治杂志,2006,13:1031-1034.
7 Bhattacharya R,Kwon J,Ali B,et al.Role of hedgehog signaling in ovarian cancer.Clin Cancer Res,2008,14:7659-7666.
8 Fujii K,Miyashita T.Hedgehog signaling pathway and human disorders.No To Hattatsu,2009,41:247-252.
9 Berman DM,Karhadkar SS,Maitra A,et al.Widespread requirement for Hedgehog ligand stimulation in growth of digestive tract tumours,Nature,2003,425:846-851.
10 Watkins DN,Berman DM,Burkhohter SG,et al.Hedgehog signalling within airway epithelial progenitors and in small-cell lung cancer.Nature,2003,422:313-317.
11 Ma X,Sheng T,Zhang Y,et al.Hedgehog signaling is activated in subsets of esophageal cancels.Int J Cancer,2006,118:139-148.
12 Nishimaki H,Kasai K,Kozaki K,et a1.A role of activated Sonic hedgehog signaling for the cellular proliferation of oral squamous cell carcinoma cell line.Biochem Biophys Res Commun,2004,314:313-320.
13 邓欢,李政,李文超,等.314例I B～ⅡA期官颈癌预后及淋巴结转移危险因素研究.中国肿瘤临床,2012,10:711-714.
14 张平,喻文人.Shh和Ptch在结肠癌、腺瘤性息肉中的表达及意义.海南医学院学报,2011,17:600-603.
15 林贞花.子宫及卵巢肿瘤病理基础研究中的几个问题.中华病理学杂志,2007,36:508-510.
16 Lin Z,Liu M,Li Z,et al.DeltaNp63 protein expression in uterine cervical and endometrial cancers.J Cancer Res Clin Oncol,2006,132:811-816.
17 Duman-Scheel M,Weng L,Xin S,et al.Hedge regulates cell growth and proliferationg by inducing Cyclin E.Nature,2002,417:299-304.