Study on the relationship between selective intrauterine growth restriction(sIUGR) and metabolic disorder-Metabonomics Study of cord blood based on gas chromatography time-of-flight mass spectrometry(GC-TOF/MS)
Zhang Xiaowei, Wang Xueju, Wei Yuan, Zhao Yangyu
Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
Abstract：Objective To investigate metabolite profiles of human umbilical cord blood in monochorionic diamnionic twin in selective intrauterine growth restriction (sIUGR) and the difference between IUGR and AGA.Methods Based on paired design, cord blood samples from sIUGR were detected by gas chromatography/time-of-flight mass spectrometry (GC-TOF/MS). Principal component analysis (PCA) and partial least squares discrimination analysis (PLS-DA) were used for data modeling. Nonparametric tests were used to test variable importance in the projection (VIP) values which exceeding 1.0 as changed metabolites.Results IUGR group and the control group were distinguished from each other in PLS-DA score plot, 10 different plasma metabolites were identified with the combination of VIP and Wilcoxon signed-rank test: sorbitol, creatine, glycine, picolinic acid were significantly increased in IUGR,while leucine, valine, palmitic acid, stearic acid, cholesterol and myo-inositol were significantly decreased (P-value<0.05).Conclusion There were differences in the composition of umbilical cord blood metabolites between sIUGR growth restricted fetus and normal fetus, which may be related to carbohydrate, amino acids and lipid metabolism abnormalities.
张晓威,王学举,魏瑗,赵扬玉. 选择性胎儿宫内生长受限与代谢异常的关系 ——基于气相色谱-飞行时间质谱的脐血代谢组学研究[J]. 中国生育健康杂志, 2017, 28(5): 421-426.
Zhang Xiaowei, Wang Xueju, Wei Yuan, Zhao Yangyu. Study on the relationship between selective intrauterine growth restriction(sIUGR) and metabolic disorder-Metabonomics Study of cord blood based on gas chromatography time-of-flight mass spectrometry(GC-TOF/MS). Chinese Journal of Reproductive Health, 2017, 28(5): 421-426.
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