Abstract：Objective To investigate the effect of walnut oligopeptides (WOPs) on improving sleep and its possible mechanism. Methods Totally 150 female specific pathogen free (SPF) BALB/c mice were randomly divided into 5 groups:a control group, a whey protein (440mg/kg BW) group, and three WOPs groups (WOPs-LG, WOPs-MG, WOPs-HG) (220, 440, 880 mg/kg BW). After 30 days of continuous intragastric administration, direct sleep test, prolonged pentobarbital sodium sleep time test, hypnotic dose of the pentobarbital sodium-induced sleeping assay and barbital sodium-induced sleeping latency test were carried out, and enzyme-linked immunosorbent assay was used to detect γ-aminobutyric acid, serotonin and dopamine content in brain. Results WOPs could not directly induce sleep. Compared with the blank control group, the sleep time of WOP-LG,WOPs-MG mice was significantly prolonged, the barbital sodium-induced sleeping latency was shortened significantly, and the contents of 5-HT and γ-aminobutyric acid in brain were significantly increased (P<0. 05). Compared with the whey protein group, the sleep time of WOPs-MG mice was significantly prolonged, the barbital sodium-induced sleeping latency was shortened significantly, and the contents of 5-HT and γ-aminobutyric acid in brain were significantly increased (P<0. 05). The intervention of whey protein and WOPs had no effect on the incidence of hypnotic dose of the pentobarbital sodium-induced sleep in mice (P>0. 05), and there was no significant difference in the content of dopamine in brain among all the groups (P>0.05). Conclusion WOPs have a certain degree of sleep improvement, and its mechanism may be related to the regulation of 5-HT, γ -aminobutyric acid in brain.
珠娜, 郝云涛, 刘欣然, 李勇. 核桃低聚肽改善睡眠作用及机制[J]. 中国生育健康杂志, 2020, 31(2): 147-150.
ZHU Na, HAO Yuntao, LIU Xinran, LI Yong. Effects of walnut oligopeptides on sleep and its mechanism. Chinese Journal of Reproductive Health, 2020, 31(2): 147-150.
