Abstract：Objective To establish a NOA (non- azoospermia) animal model with different degrees of injury by means of busulfan to the reproductive toxicity of BALB/ C male mice. Methods A total of 45 BALB/ C male mice aged 6-8 weeks were randomly divided into 3 groups:0 mg/kg group, 30 mg/kg group and 40 mg/kg group, 15 mice in each group. Weight of mice, wet weight of testis and epididymis, testicular volume, and spermatogenic cell damage were observed and recorded every 2 weeks. The damage of spermatogenic cells was determined by HE staining. By immunohistochemical technology to detect damage at the stagnation of sperm cells meiosis stage, it mainly uses three of the born sperm cells meiosis marker Protein:STRA8 (Stimulated by Retinoic Acid, 8 STRA8) meiosis start marker Protein, SCP3 (Synaptonemal Complex Protein 3, SCP3) marker Protein in meiosis, TNP1 (Transition Protein 1, TNP1) marker Protein after meiosis. Results The mice survived well after the injection. After 2 weeks of the injection, spermatogenic cells began to damage, and the damage in the 30 mg/kg group was less than that in the 40 mg/kg group. At 4 weeks, the 40 mg/kg group showed only supportive cell changes. At 6-10 weeks, the spermatogenic function began to recover, and the recovery was significantly faster in the 30 mg/kg group than in the 40 mg/kg group. The expression of STRA8, SCP3 and TNP1 in the 40 mg/kg group was lowest at 4 weeks after injection, and returned to normal at 8 weeks. Conclusion After a single injection of 40 mg/kg baishangan for 4 weeks, the model of NOA spermatogenesis disturbance could be established. During 4-8 weeks of injection, NOA spermatogenic disorder models with different damage degrees could be established, providing stable research tissues for testicular tissue culture in vitro.
李慧赟, 王慧芳, 孟晓丽, 赵均, 宋春英, 郭兴萍. 利用白消安致BALB/c小鼠生殖毒性建立NOA疾病模型[J]. 中国生育健康杂志, 2021, 32(3): 247-251.
LI Huiyun, WANG Huifang, MENG Xiaoli, ZHAO Jun, SONG Chunying, GUO Xingping. Establishment of NOA Model in BALB/c mice induced by busulfan. Chinese Journal of Reproductive Health, 2021, 32(3): 247-251.
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