Abstract：Objective To study the developmental toxicity of genetically modified maize Bt799 and the Cry1Ac protein in it by culturing rat embryos palatal plate and midbrain cells in vitro.Methods Wistar rats, aged 9 weeks, weighing (200±20)g, were purchased from the Experimental Animal Department of Peking University Health Science Center and raised in SPF animal room. The first part:The embryos midbrain cells were extracted on the 13th day of gestation, after 5 days of culture under different conditions(the fetal bovine serum was replaced by immediate centrifugation serum), the proliferation and differentiation of midbrain cells were observed. The culture of midbrain cells was divided into eight groups, corresponding to eight different culture environments. As source of ICS, the Wistar rats were randomly divided into six groups. The first five groups included NM group, TM3 group, TM1M group, TM3M group and PCB group; BP group, BC group and PCA group used the serum of the sixth group together because the rats had no direct intervention and it did not affect the comparison between groups. The TM3, TM1M and TM3M groups were treated with the serum of adult rats after intervention with Bt799 corn for different duration(1 day, 1 month, 3 months), BP group with the serum supplemented with Cry1Ac protein, PCA group with the serum supplemented with bisphenol A, PCB group with the serum extracted from rats perfused with bisphenol A for 3 days, NM group with the serum of the rats Intervened by the parent maize Zheng 58 for 3 days, and BC group with the serum of rats fed with AIN-93G diet for 3 days. The second part:On the 12.5th day of gestation, the palatal plates of rat embryos were extracted and then cultured for 72 hours by adding Cry1Ac protein(BP group) or all-trans retinoic acid(RA group). The palatal plate fusion rate was calculated to determine the effect of Cry1Ac protein on the development of the palatal plate of the rat embryos.Results The first part:The proliferation rate and differentiation rate in TM3, TM1M, TM3M and BP groups were not significantly different from those in NM group and BC group(P>0.05). Compared with NM group and BC group, the proliferation rate and differentiation rate in PCA group and PCB group significantly decreased(P<0.05). The second part:There was no significant difference in palatal fusion rate between BP group and BC group(P>0.05). Compared with BC group, the palatal fusion rate of RA significantly decreased(P<0.05).Conclusion No embryo-developmental toxicity of Bt799 maize and Cry1Ac protein was observed in this study.
侯超, 郭倩颖, 刘欣然, 刘思奇, 麻慧娟, 刘珊, 王军波. 基因工程玉米Bt799对大鼠胚胎中脑细胞微团及腭板发育的影响[J]. 中国生育健康杂志, 2021, 32(4): 343-349.
HOU Chao, GUO Qianying, LIU Xinran, LIU Siqi, MA Huijuan, LIU Shan, WANG Junbo. Effect of Bt799 maize on development of embryos midbrain cell cultures and palate in rats. Chinese Journal of Reproductive Health, 2021, 32(4): 343-349.
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