The association between serum 8-hydroxy-2′-deoxyguanosine exposure and hypertensive disorders of pregnancy

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Chinese Journal of Reproductive Health ›› 2024, Vol. 35 ›› Issue (5) : 401-407.

JIN Ming, LIU Xiaojing, LIU Xiaowen, ZHANG Ziyi, GAO Suhong, WU Yaxian, CHEN Junxi, ZHANG Le, YE Rongwei, LIU Xiaohong, LI Zhiwen, LI Nan

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Abstract

Objective This study aims to investigate the association between 8-hydroxydeoxyguanosine(8-OHdG) in serum and the incidence of hypertensive disorders of pregnancy(HDP). Methods This nested case-control study was based on a cohort of pregnant women at the Haidian Maternal and Child Health Hospital in Beijing. The cohort recruited pregnant women from October 2017 to October 2018. The study ultimately included 150 HDP cases and 490 healthy controls. General demographic characteristics and lifestyle information were collected through structured face-to-face questionnaires during baseline prenatal visits, and leftover blood samples were retained from laboratory tests. The concentration of 8-OHdG in the serum of pregnant women was measured using an ELISA kit. HDP was diagnosed based on the first occurrence of hypertension after 20 weeks of pregnancy. Logistic regression was used to analyze the association between serum 8-OHdG and the risk of HDP, calculating the odds ratio(OR) and 95% confidence interval(CI). Further exploration of the dose-response relationship between serum 8-OHdG and HDP, as well as the potential interaction between pre-pregnancy body mass index(BMI) and serum 8-OHdG on the incidence of HDP, was conducted. Results In this study, the detection rate of serum 8-OHdG in pregnant women was 93.59%, with a median concentration of 28.98 ng/mL. Logistic regression results showed that high exposure to serum 8-OHdG had a significant protective effect against HDP. When serum 8-OHdG was divided into quartiles, the risk of HDP in the high exposure group(Q4) was 0.42 times that of the low exposure group(Q1)(95%CI:0.22, 0.78), and there was a significant dose-response relationship(linear trend test P=0.001). High exposure to 8-OHdG significantly reduced the risk of HDP only in pregnant women with a pre-pregnancy BMI ≥ 24 kg/m², and there was a significant interaction between pre-pregnancy BMI and serum 8-OHdG on the incidence of HDP(P=0.012). Conclusion Exposure to serum 8-OHdG during pregnancy can reduce the risk of HDP, and pre-pregnancy BMI can modify this association.

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hypertensive disorders of pregnancy / 8-hydroxy-2′-deoxyguanosine / pre-pregnancy body mass index / nested case-control study

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JIN Ming, LIU Xiaojing, LIU Xiaowen, ZHANG Ziyi, GAO Suhong, WU Yaxian, CHEN Junxi, ZHANG Le, YE Rongwei, LIU Xiaohong, LI Zhiwen, LI Nan. The association between serum 8-hydroxy-2′-deoxyguanosine exposure and hypertensive disorders of pregnancy[J]. Chinese Journal of Reproductive Health, 2024, 35(5): 401-407

References

1 Li N,Li Z,Ye R,et al.Preconception blood pressure and risk of preterm birth:a large cohort study in China.J Hypertens,2016,34:2243-2247.
2 Li N,Li Z,Ye R,et al.Preconception blood pressure and Risk of low birth weight and small for gestational age:a large cohort study in China.Hypertension,2016,68:873-879.
3 Henry A,Canoy D.Editorial:Hypertension during pregnancy and future risk of cardiovascular and other long-term health outcomes.Front Cardiovasc Med,2020,7:569735.
4 Kajantie E,Eriksson JG,Osmond C,et al.Pre-eclampsia is associated with increased risk of stroke in the adult offspring:the Helsinki birth cohort study.Stroke,2009,40:1176-1180.
5 Umesawa M,Kobashi G.Epidemiology of hypertensive disorders in pregnancy:prevalence,risk factors,predictors and prognosis.Hypertens Res,2017,40:213-220.
6 李丽,付强强.中国妊娠期高血压疾病患病率的Meta分析.中国妇幼保健,2019,34:3378-3381.
7 Li F,Qin J,Zhang S,et al.Prevalence of hypertensive disorders in pregnancy in China:A systematic review and meta-analysis.Pregnancy Hypertens,2021,24:13-21.
8 Ye C,Ruan Y,Zou L,et al.The 2011 survey on hypertensive disorders of pregnancy(HDP) in China:prevalence,risk factors,complications,pregnancy and perinatal outcomes.PLoS One,2014,9:e100180.
9 Chiarello DI,Abad C,Rojas D,et al.Oxidative stress:Normal pregnancy versus preeclampsia.Biochim Biophys Acta Mol Basis Dis,2020,1866:165354.
10 Akinci S,Özcan HC,Balat Ö,et al.Assessment of β-hydroxydeoxyguanosine levels in patients with preeclampsia:a prospective study.Clin Exp Obstet Gynecol,2017,44:226-229.
11 Toljic M,Egic A,Munjas J,et al.Increased oxidative stress and cytokinesis-block micronucleus cytome assay parameters in pregnant women with gestational diabetes mellitus and gestational arterial hypertension.Reprod Toxicol,2017,71:55-62.
12 Fukushima K,Murata M,Tsukimori K,et al.8-Hydroxy-2-deoxyguanosine staining in placenta is associated with maternal serum uric acid levels and gestational age at diagnosis in pre-eclampsia.Am J Hypertens,2011,24:829-834.
13 Ferguson KK,Meeker JD,McElrath TF,et al.Repeated measures of inflammation and oxidative stress biomarkers in preeclamptic and normotensive pregnancies.Am J Obstet Gynecol,2017,216:527 e521-527 e529.
14 Anto EO,Coall DA,Addai-Mensah O,et al.Early gestational profiling of oxidative stress and angiogenic growth mediators as predictive,preventive and personalised(3P) medical approach to identify suboptimal health pregnant mothers likely to develop preeclampsia.EPMA J,2021,12:517-534.
15 Savitri AI,Zuithoff P,Browne JL,et al.Does pre-pregnancy BMI determine blood pressure during pregnancy? A prospective cohort study.BMJ Open,2016,6:e011626.
16 Zavalza-Gómez AB.Obesity and oxidative stress:a direct link to preeclampsia.Arch Gynecol Obstet,2011,283:415-422.
17 中华医学会妇产科学分会妊娠期高血压疾病学组.妊娠期高血压疾病诊治指南(2020).中华妇产科杂志,2020,55:227-238.
18 梁瑞英,叶荣伟,李宏田,等.浙江省嘉兴地区初孕妇女自然流产状况研究.中华流行病学杂志,2010,31:755-758.
19 Zhou A,Xiong C,Hu R,et al.Pre-pregnancy BMI,gestational weight gain,and the risk of hypertensive disorders of pregnancy:a cohort study in Wuhan,China.PLoS One,2015,10:e0136291.
20 Stein PT,Scholl TO,Schluter MD,et al.Oxidative stress early in pregnancy and pregnancy outcome.Free Radic Res,2008,42:841-848.
21 Bruner SD,Norman DP,Verdine GL.Structural basis for recognition and repair of the endogenous mutagen 8-oxoguanine in DNA.Nature,2000,403:859-866.
22 Cejvanovic V,Asferg C,Kj?r LK,et al.Markers of oxidative stress in obese men with and without hypertension.Scand J Clin Lab Invest,2016,76:620-625.
23 Hsieh TT,Chen SF,Lo LM,et al.The association between maternal oxidative stress at mid-gestation and subsequent pregnancy complications.Reprod Sci,2012,19:505-512.
24 Negi R,Pande D,Karki K,et al.Association of oxidative DNA damage,protein oxidation and antioxidant function with oxidative stress induced cellular injury in pre-eclamptic/eclamptic mothers during fetal circulation.Chem Biol Interact,2014,208:77-83.
25 Owaki Y,Watanabe K,Iwasaki A,et al.Placental hypoplasia and maternal organic vascular disorder in pregnant women with gestational hypertension and preeclampsia.J Matern Fetal Neonatal Med,2021,34:353-359.
26 Nobles CJ,Williams A,Ouidir M,et al.Differential Effect of ambient air pollution exposure on risk of gestational hypertension and preeclampsia.Hypertension,2019,74:384-390.
27 Ferguson KK,McElrath TF,Chen YH,et al.Repeated measures of urinary oxidative stress biomarkers during pregnancy and preterm birth.Am J Obstet Gynecol,2015,212:208.e1-8.
28 Huh JY,Jung I,Piao L,et al.8-Hydroxy-2-deoxyguanosine ameliorates high-fat diet-induced insulin resistance and adipocyte dysfunction in mice.Biochem Biophys Res Commun,2017,491:890-896.