Abstract：Objective To examine the effect of levonorgestrel-loaded nanoparticle composites on embryo implantation in vitro. Methods We detected the Bewo cell spheroids’ adhesion rate and spreading rate as well as the mRNA and protein of integrin αv in the control group, the empty biofilm group and drug-loaded biofilm group. Results Compared with control group and the empty biofilm group, the drug-loaded biofilm group showed obvious difference in Bewo cell spheroids’ adhesion rate and spreading rate as well as the expression level of mRNA and protein of integrin αv of Ishikawa cells. Compared with the control group, the empty biofilm group and the drug-loaded biofilm group had no effect on proliferation of Ishikawa cells. Conclusion Polylactic acid-polystyrene composites have no effect on embryo implantation, while levonorgestrel-loaded nanoparticle polylactic acid-polystyrene composites affect embryo implantation in vitro. The data provide a theoretical basis for biological safety of empty biofilm and effectiveness of drug-loaded biofilm, and for levonorgestrel-loaded nanoparticle polylactic acid-polystyrene composites in clinical application as a new intrauterine contraceptive system.
王品, 顾方乐, 魏勉, 黄夏曼, 李媛媛, 王海燕, 潘宇, 张晓梅. 左炔诺孕酮纳米载药缓释复合材料对体外胚胎着床影响的实验研究[J]. 中国生育健康杂志, 2015, 26(5): 401-403.
WANG Pin, GU Fangle, WEI mian, HUANG Xiaman, LI Yuanyuan, WANG Haiyan, PAN Yu, ZHANG Xiaomei. Experimental research of the effects of sustained-released levonorgestrel nanoparticle composites on embryo implantation in vitro. Chinese Journal of Reproductive Health, 2015, 26(5): 401-403.
1 嵇玉蓉,魏勉,张晓梅.生物可降解纳米载药缓释宫内节育系统的构建及应用前景.中国医药导刊,2012,14:2025-2027. 2 张晓梅,王大新,吕芳,等.生物可降解纳米载药缓释宫内节育器及其制备方法.中国.201310008740.5[P].2013-4-17. 3 徐亚雷,侯连龙,夏鹏.聚乳酸高性能化的研究进展.塑料工业,2012,40:14-18. 4 林丽丽.聚乳酸及其共聚物在生物相容性方面的探讨.发酵科技通讯,2013,42:51-55. 5 Louwen JN,Stedeford T.Computational assessment of the environmental fate,bioaccumulation,and toxicity potential of brominated benzylpolystyrene.Toxicol Mech Methods,2011,21:183-192.6 Tahir L,Ishtiaq Ali M,Zia M, et al.Production and characterization of esterase in Lantinus tigrinus for degradation of polystyrene.Pol J Microbiol,2013,62:101-108. 7 李德玲,王丽红,刘会员,等.聚乳酸/聚苯乙烯共混物的热氧稳定性研究.化学研究与应用,2011,23:1334-1336. 8 Yang Z,Kang SG,Zhou R.Nanomedicine: de novo design of nanodrugs.Nanoscale,2014,21:663-677. 9 Matsumura Y.The drug discovery by nanomedicine and its clinical experience.Jpn J Clin Oncol,2014,44:515-525. 10 周雅轩.纳米载药系统在医药领域中的应用进展.天津药学,2012,24:47-49. 11 Granicka LH.Nanoencapsulation of cells within multilayer shells for biomedical applications.J Nanosci Nanotechnol,2014,14:705-716. 12 Lee WH,Loo CY,Young PM,et al.Recent advances in curcumin nanoformulation for cancer therapy.Expert Opin Drug Deliv,2014,11:1183-1201. 13 Gao H,Pang Z,Jiang X.Targeted delivery of nano-therapeutics for major disorders of the central nervous system.Pharm Res,2013,30:2485-2498.
