Effects of sub-chronic exposure to BPA on ovarian reserve function in female mice
CAO Yuming, QU Xinlan, ZHANG Ming,ZUO Chi, YAO Yanru, YANG Hanxiao, ZHANG Yuanzhen
Department of Obstetrics and Gynecology & Reproductive Medicine Center, Zhongnan Hospital of Wuhan University; Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan 430071, China
Abstract：Objective To investigate whether sub-chronic oral exposure to low-dose BPA can affect ovarian reserve in female mice.Methods Sixty-four 5-week-old SPF C57BL/6 mice were randomly divided into 4 groups, exposed to 5, 50, and 500ug/ml of BPA solution, respectively, and the control group was fed with corn oil alone. After 28 days, blood samples were drawn from the orbital vein of each mice, and the AMH and E2 hormone concentration in serum was detected by ELISA kit. Mice were sacrificed by cervical dislocation, and ovaries were removed immediately. The expression of AMH mRNA was detected by real-time quantitative PCR. The expression of AMH protein was detected by immunohistochemistry.Results Compared with the control group, the level AMH and E2, the expression of AMH gene and protein in the BPA treatment group showed a downward trend. The low dose group and high dose group decreased significantly (P<0.05).Conclusion There was no single dose effect of BPA on mouse ovary, and sub-chronic exposure to low dose of BPA could induce the decrease of ovarian reserve function in female mice.
曹羽明,瞿鑫兰,张铭,左驰,姚燕如,杨寒笑,张元珍. 双酚A亚慢性暴露对雌性小鼠卵巢储备功能的影响[J]. 中国生育健康杂志, 2017, 28(3): 212-216.
CAO Yuming, QU Xinlan, ZHANG Ming,ZUO Chi, YAO Yanru, YANG Hanxiao, ZHANG Yuanzhen. Effects of sub-chronic exposure to BPA on ovarian reserve function in female mice. Chinese Journal of Reproductive Health, 2017, 28(3): 212-216.
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