Abstract：Objective To examine germ cell apoptosis in rat induced by testicular hyperthermia, and the effect of Fas/FasL signal pathway on apoptosis induction.Methods Sixteen adult male rats were randomly divided into heating group and control group. Rats in the heating group were treated with local testicular short-term hyperthermia exposure in water bath at 43℃ for 15 min. Rats in the control group were treated as the same above but the water temperature was 22℃. After 24 hours, perfusion was done for intratissue fixation. The testes of two groups were taken and prepared in 5 micrometers thick slices. Germ cell apoptosis was evaluated by TUNEL, and the expression of Fas and FasL was analyzed by immunohistochemistry. Serum testosterone levels in the rats was detected by chemiluminescence.Results Compared to the control group, index of germ cell apoptosis in the heating group was significantly higher (15.8%±1.6% vs 2.2%±0.5%, P<0.01). Fas and Fas-L expression increased markedly in germ cell and sertoli cell in the heating group. No difference was found in serum testosterone levels between the two groups.Conclusion Testicular hyperthermia exposure induces germ cell apoptosis; Fas/FasL signal pathway is involved in this apoptosis process.
王雪松, 曹龙巧, 乔岩岩, 孙秀芹. Fas/FasL参与热诱导大鼠睾丸生精细胞凋亡的调控[J]. 中国生育健康杂志, 2014, 25(4): 326-328.
WANG Xuesong, CAO Longqiao, QIAO Yanyan, SUN Xiuqin. Fas and FasL involved in apoptosis of rat germ cell induced by heat exposure. Chinese Journal of Reproductive Health, 2014, 25(4): 326-328.
1 郝丽荣,安长新.睾丸温度升高对男性生殖功能的影响.中国男科学杂志,2009,11:70-72. 2王如刚,张非若,陶永娴.职业危害因素对男性生殖健康影响的研究进展.中华劳动卫生职业病杂志,2013,2:142-145. 3朱启星,主编.卫生学.第8版.北京:人民卫生出版社,2013:360-361. 4王旭初,罗莉,刘启瑞,等.多囊卵巢综合征合并慢性盆腔炎不孕症188例临床分析.中国生育健康杂志,2013,1:49-50. 5李宏军,李汉忠.严格掌握男性不育患者精索静脉曲张的手术适应证.中华泌尿外科杂志,2010,4:221-222. 6Sinha Hikim AP,Lue Y,Diaz Romero M,et al.Deciphering the pathways of germ cell apoptosis in the testis.J Steroid Mol Biol,2003,85:175-182. 7Villa-Morales M,Fernández-Piqueras J.Targeting the Fas/FasL signaling pathway in cancer therapy.Expert Opin Ther Targets,2012,16:85-101. 8智二磊,徐计秀,王璟琦,等.血清抗精子抗体阳性对青春期雄性大鼠睾丸组织及睾丸生殖细胞中Fas/Fas-L凋亡途径的影响.中华男科学杂志,2013,19:111-115. 9Hayashi T,Yoshida S,Yoshinaga A,et al.HtrA2 is up-regulated in the rat testis after experimental cryptorchidism.Int J Urol,2006,13:157-164. 10Li L,Han ZY,Li CM,et al.Upregulation of heat shock protein 32 in Sertoli cells alleviates the impairments caused by heat shock-induced apoptosis in mouse testis.Cell Stress Chaperon,2013,18:333-351. 11Li Y,Huang Y,Piao Y,et al.Protective effects of nuclear factor erythroid 2-related factor 2 on whole body heat stress-induced oxidative damage in the mouse testis.Reprod Biol Endocrinol,2013,11:23. 12Vera Y,Diaz-Romero M,Rodriguez S,et al.Mitochondria-dependent pathway is involved in heat-induced male germ cell death:lessons from mutant mice.Biol Reprod,2004,70:1534-1540. 13Absalan F,Movahedin M,Mowla SJ.Evaluation of apoptotic genes expression and its protein after treatment of cryptorchid mice.Iran Biomed J,2012,16:77-83. 14Kheradmand A,Dezfoulian O,Alirezaei M.Ghrelin regulates Bax and PCNA but not Bcl-2 expressions following scrotal hyperthermia in the rat.Tissue Cell,2012,44:308-315. 15Mostafa T,Anis T,Imam H,et al.Seminal reactive oxygen species-antioxidant relationship in fertile males with and without varicocele.Andrologia,2009,2:125-129. 16Xue Juan,Yang Jianfu,Yan Jin,et al.Abnormalities of the testes and semen parameters in clinical varicocele.J South Med Univ,2011,4:439-442. 17Ahmad G,Moinard N,Esquerr -Lamare C,et al.Mild induced testicular and epididymal hyperthermia alters sperm chromatin integrity in men.Fertil Steril,2012,97:546-553.
