Abstract：Objective ObjectiveTo investigate the potential effect of BMP7 abnormal changes on granulosa cell proliferation.Methods A total of 44 PCOS patients (PCOS group) and 11 female patients with normal ovarian function (control group) we enrolled. General information, WHR, age, FSH, Gn dosage, and BMI were recorded. Real-time PCR was used to detect the mRNA levels of BMP7, Bcl-2 and Caspase-3 in granulosa cells. Western blotting was used to detect the expression of BMP7 in granulosa cells. The apoptosis index of granulosa cell was detected by flow cytometry.Results Compared with the control group, age, serum FSH and Gn dosage levels were significantly decreased, bur BMI, LH, E2, and T were increased in the PCOS group. BMP7 and Bcl-2 expression was significantly decreased in the PCOS group compared with the control group. However, the apoptosis index was significantly increased in the PCOS group compared with the control group.Conclusion Abnormal changes in BMP7 may affect oocyte quality and embryo development potential, and may be correlated with the granulosa cell apoptosis.
刘俊芬,崔向荣,吴媛霞,苏丹,武学清. 多囊卵巢综合征患者BMP-7异常表达对颗粒细胞增殖的影响[J]. 中国生育健康杂志, 2017, 28(1): 13-16.
LIU Junfen, CUI Xiangrong, WU Yuanxia, SU Dan, WU Xueqing. The potential effect of BMP7 abnormal expression on granulosa cell proliferation in patients with PCOS. Chinese Journal of Reproductive Health, 2017, 28(1): 13-16.
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