Abstract：Objective To investigate the potential effect of CENP-H abnormal changes on chromosomal numerical abnormality in spontaneous abortion embryos. Methods Fluorescence in situ hybridization was used to detect the numerical chromosome abnormality in 96 villus tissues from women with spontaneous abortion. Real-time PCR and western-blotting were used to detect the mRNA and protein levels of CENP-H in the villus tissues of all subjects.Results 44.79% of the cases had numerical chromosome abnormality, including autosomal abnormalities (26 cases) and sex chromosomal abnormalities (10 cases). Compared with normal chromosome group, the mRNA and protein levels of CENP-H were significantly decreased in chromosome abnormality group (P<0.05).Conclusion From these data we supposed that the abnormal changes of CENP-H may be correlated with chromosomal numerical abnormality in the villus tissues of spontaneous abortion embryos.
井宣, 崔向荣, 霍凯, 义建伟, 李晓芳, 刘荣华. 自然流产胚胎绒毛中着丝粒蛋白-H的异常表达与染色体数目异常的相关性研究[J]. 中国生育健康杂志, 2016, 27(4): 331-334.
JING Xuan, CUI Xiangrong, HUO Kai, YI Jianwe, LI Xiaofang, YAN Meiqin. Potential effect of CENP-H abnormal changes on chromosomal numerical abnormality in spontaneous abortion embryos. Chinese Journal of Reproductive Health, 2016, 27(4): 331-334.
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