Abstract：Objective To investigate the relationship between maternal-fetal LMP and HLA-DM gene sharing rate and the incidence of pregnancy-induced hypertension in Hainan. Methods A total of 60 mothers and fetuses with pregnancy-induced hypertension who gave birth in the obstetrics department of our hospital from August 2016 to August 2018 were selected. 200 pairs of healthy pregnant women and their fetuses who gave birth in our hospital during the same period were selected as the control group. Blood samples were collected from all subjects, and DNA from peripheral blood leukocytes was extracted. Then PCR amplification followed by gene sequencing. To compare the difference in the sharing rate of LMP and HLA-DM between cases with pregnancy-induced hypertension and healthy controls. Results When HLA-DM was shared by two, the proportion of hypertensive disease group during pregnancy was significantly higher than that of the controls (P<0.01). When the LMP was shared by 4, the proportion of hypertension in pregnancy was significantly higher than that of the controlse (P<0.01). The HLA-DMB* 0103 locus and LMP2-R/H gene sharing rate was the highest in the patients with hypertension in pregnancy, which was significantly different from the control group (P<0.05). There was no significant difference in the sharing rate of other sites (P>0.05). Conclusion The incidence of hypertension during pregnancy in Hainan may be related to sharing rate of LMP and HLA-DM genes among women with pregnancy-induced hypertension and their fetus in Hainan. Among them, HLA-DMB* 0103 and LMP2-R/H may be susceptible genes for pregnancy-induced hypertension.
韩贞艳, 陈建林, 关红琼. 海南地区妊娠期高血压疾病患者母胎LMP和HLA-DM基因共享率研究[J]. 中国生育健康杂志, 2021, 32(3): 225-228.
HAN Zhenyan, CHEN Jianlin, GUAN Hongqiong. Association on the sharing rate of maternal fetal LMP and HLA-DM genes in women with pregnancy-induced hypertension in Hainan. Chinese Journal of Reproductive Health, 2021, 32(3): 225-228.
1 Lopez GCA,Mitchell BM.Recent Advances in Immunity and Hypertension.Am J Hypertens,2017,30:643-652. 2 Persson G,Melsted WN,Nilsson LL,et al.HLA class Ib in pregnancy and pregnancy-related disorders.Immunogenetics,2017,69:581-595. 3 Kloesges T,Popa O,Martin W,et al.Networks of gene sharing among 329 proteobacterial genomes reveal differences in lateral gene transfer frequency at different phylogenetic depths.Mol Biol Evol,2011,28:1057-1074. 4 Agius A,Sultana R,Camenzuli C,et al.An update on the genetics of pre-eclampsia.Minerva Ginecol,2018,70:465-479. 5 王香林,赵得雄,庄文婷,等.基因多态性与青海妊娠期高血压疾病的相关性.解剖学报,2018,49:379-386. 6 中华医学会妇产科学分会妊娠期高血压疾病学组.妊娠期高血压疾病诊治指南(2015).中华产科急救电子杂志,2015,4:206-213. 7 Mellins ED,Stern LJ.HLA-DM and HLA-DO,key regulators of MHC-II processing and presentation.Curr Opin Immunol,2014,26:115-122. 8 朱薏,高眉扬.HLA-DM基因夫妇共享率与子痫前期相关性.实用医学杂志,2007,23:2645-2646. 9 Dosenko V,Mykhalchuk DV,Zahoriy V,et al.Allelic polymorphism of genes encoding catalytic immunoproteasome subunits and its functional meaning.Fiziol Zh,2005,51:3-10. 10 张嵩卉,高眉扬,田耕,等.LMP 基因多态性及其在母胎间共享与子痫前期相关性研究.现代妇产科进展,2012,21:175-177.
