视黄酸通过miR-210促进隐睾生精细胞增殖分化

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摘要目的探讨视黄酸(RA)通过miR-210对隐睾生精细胞增殖分化的影响。方法使用氟他胺诱导隐睾小鼠模型,实验分为:正常对照组、氟他胺组、RA治疗组、miR-210敲降组、miR-210过表达组以及miR-210过表达+RA治疗组,每组各10只。使用RT-qPCR检测miR-210、C-kit和PLZF mRNA的表达水平。使用Western blot检测C-kit和PLZF的表达水平。结果 RA可显著上调隐睾组织中C-kit、PLZF mRNA和蛋白的表达水平,下调miR-210的表达水平。敲降miR-210可上调隐睾组织中C-kit、PLZF mRNA和蛋白表达水平,而过表达miR-210则具有相反的结果;同时,过表达miR-210+RA治疗可恢复过表达miR-210对C-kit和PLZF表达的抑制作用。结论 miR-210在隐睾组织中高表达,RA可通过下调miR-210的表达水平促进隐睾生精细胞增殖分化。

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	陆峰
	安妮妮
	陈辉
	何国庆
	占雄
	吴谋东
	汪丹
	王蔚
	彭金普

关键词 ：隐睾症, 生精细胞, 增殖分化, 视黄酸, miR-210

Abstract：Objective To discuss the effect of retinoic acid(RA) on proliferation and differentiation of cryptorchidism spermatogenic cells via miR-210.Methods To establish a model of flutamide-induced cryptorchidism mouse. The experiment was divided into normal control group, flutamide group, RA treatment group, miR-210 knockdown group, miR-210 overexpression group and miR-210 overexpression + RA treatment group. RT-qPCR was performed to assess the expression level of miR-210, C-kit and PLZF mRNA. Western blot was employed to detect the expression level of C-kit and PLZF protein.Results RA significantly upregulated the expression levels of C-kit and PLZF mRNA and protein in cryptorchidism tissues, and downregulated the expression levels of miR-210. Knockdown of miR-210 could upregulate the expression of C-kit and PLZF mRNA and protein in cryptorchidism tissues, while overexpression of miR-210 had the opposite result. At the same time, miR-210 overexpression + RA treatment could restore the inhibition of miR-210 overexpression on the expression of C-kit and PLZF.Conclusion miR-210 is highly expressed in cryptorchidism tissues, and RA can promote the proliferation and differentiation of cryptorchidism spermatogenic cells by downregulating the expression level of miR-210.

Key words： cryptorchidism spermatogenic cell proliferation and differentiation retinoic acid miR-210

收稿日期: 2020-07-09

ZTFLH:

R71

基金资助:贵州省卫生计生委科学技术基金项目(gzwjkj20177-1-035)

通讯作者: 彭金普(pink-floyd@foxmail.com)

引用本文:

陆峰, 安妮妮, 陈辉, 何国庆, 占雄, 吴谋东, 汪丹, 王蔚, 彭金普. 视黄酸通过miR-210促进隐睾生精细胞增殖分化[J]. 中国生育健康杂志, 2022, 33(5): 439-443. LU Feng, AN Nini, CHEN Hui, HE Guoqing, ZHAN Xiong, WU Moudong, WANG Dan, WANG Wei, PENG Jinpu. Retinoic acid promoted proliferation and differentiation of cryptorchidism spermatogenic cells via miR-210. Chinese Journal of Reproductive Health, 2022, 33(5): 439-443.

链接本文:

http://cjrh.bjmu.edu.cn/CN/ 或 http://cjrh.bjmu.edu.cn/CN/Y2022/V33/I5/439

1 Lee PA,Houk CP.Cryptorchidism.Curr Opin Endocrinol Diabetes Obes,2013,20:210-216.
2 Yu CJ,Long CL,Wei Y,et al.Evaluation of Fowler-Stephens orchiopexy for high-level intra-abdominal cryptorchidism:A systematic review and meta-analysis.Int J Surg,2018,60:74-87.
3 Chua ME,Mendoza JS,Gaston MJV,et al.Hormonal therapy using gonadotropin releasing hormone for improvement of fertility index among children with cryptorchidism:A meta-analysis and systematic review.J Pediatr Surg,2014,49:1659-1667.
4 Balmer JE,Blomhoff R.Gene expression regulation by retinoic acid.J Lipid Res,2002,43:1773-1808.
5 Li X,Long XY,Xie YJ,et al.The roles of retinoic acid in the differentiation of spermatogonia and spermatogenic disorders.Clin Chim Acta,2019,497:54-60.
6 Heidary Z,Zaki-Dizaji M,Saliminejad K,et al.MiR-4485-3p expression reduced in spermatozoa of men with idiopathic asthenozoospermia.Andrologia,2020,52:e13539.
7 Sun JX,Niu L,Gao SX,et al.MiR-509-5p downregulation is associated with male infertility and acts as a suppressor in testicular germ cell tumor cells through targeting MDM2.Onco Targets Ther,2019,12:10515-10522.
8 Perri M,Caroleo MC,Liu NN,et al.9-cis Retinoic acid modulates myotrophin expression and its miR in physiological and pathophysiological cell models.Exp Cell Res,2017,354:25-30.
9 Wu HB,Zhao JM,Fu BB,et al.Retinoic acid-induced upregulation of miR-219 promotes the differentiation of embryonic stem cells into neural cells.Cell Death Dis,2017,8:e2953.
10 Hermann BP,Chen KR,Singh A,et al.The mammalian spermatogenesis single-cell transcriptome,from spermatogonial stem cells to spermatids.Cell Rep,2018,25:1650-1667. e1658.
11 Sharma M,Srivastava A,Fairfield HE,et al.Identification of EOMES-expressing spermatogonial stem cells and their regulation by PLZF.Elife,2019,8.
12 Tang RL,Fan LQ.PLZF(pos)c-KIT(pos)-delineated A1-A4-differentiating spermatogonia by subset and stage detection upon Bouin fixation.Asian J Androl,2019,21:309-318.
13 Wang JJ,Li JM,Xu W,et al.Androgen promotes differentiation of PLZF(+) spermatogonia pool via indirect regulatory pattern.Cell Commun Signal,2019,17:57.
14 Lee R,Lee WY,Park HJ,et al.Stage-specific expression of DDX4 and c-kit at different developmental stages of the porcine testis.Anim Reprod Sci,2018,190:18-26.
15 Unni S K,Modi DN,Pathak SG,et al.Stage-specific localization and expression of c-kit in the adult human testis.J Histochem Cytochem,2009,57:861-869.
16 Lu TX,Rothenberg ME.MicroRNA.J Allergy Clin Immunol,2018,141:1202-1207.
17 Yadav SK,Pandey A,Kumar L,et al.The thermo-sensitive gene expression signatures of spermatogenesis.Reprod Biol Endocrinol,2018,16:56; DOI:10. 1186/s12958-018-0372-8.
18 Huang ZY,Tang DD,Gao JJ,et al.miR-34c disrupts spermatogonial stem cell homeostasis in cryptorchid testes by targeting Nanos2. Reprod Biol Endocrinol,2018,16:97.
19 Moritoki Y,Hayashi Y,Mizuno K,et al.Expression profiling of microRNA in cryptorchid testes:miR-135a contributes to the maintenance of spermatogonial stem cells by regulating FoxO1.J Urol,2014,191:1174-1180.