Abstract：Objective This study aimed to investigate the potential impact of mouse blastocyst vitrification on offspring growth, nerve function, behavior and fertility to assess the long-term safety of this technology.Methods Premature C57BL/6 female mice were superovulated. Blastocysts were collected, vitrified and transplanted into ICR surrogate mouse. After the pups were born, litter size and abnormalities, if any, were observed. Physiological development indices at different observation points were recorded. Rotarod test, water maze and social behavior test were done to check motion coordination, learning and memory function and social behavior. Fertility test was carried out when male mouse reached 8 weeks of age.Results Our results showed that there were no statistical difference in any of the development indicators, behavior tests and social ability compare to control mice. Blastocyst vitrification male mice fertility and litter size were 37.5 % and 6.7 ± 2.5, respectively, comparable to 35.0% and 6.5 ± 1.9, respectively, in the control group.Conclusion Blastocyst vitrification has no impact on mouse growth, behavioral characteristics and male fertility, suggesting that blastocyst vitrification is a safe and reliable technique for embryo cryopreservation.
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