Effects of maternal exposure to PM2.5 during pregnancy and lactation period on growth and development of offspring mice and the protective effects of VC and quercetin
ZHANG Minjia, LIU Wei, ZHOU Yalin, FENG Jinqiu, FAN Aiqin, LI Yong, XU Yajun
Department of Nutrition and Food Hygiene, Peking University, Beijing 100191, China
Abstract:Objective To study the effects of PM2.5 exposure on the growth and development of offspring mice during pregnancy and lactation, and to investigate whether VC and quercetin can improve the growth and development of mice exposed to PM2.5 during pregnancy and lactation.Methods PM2.5 samples were collected from an area in Beijing during the non-heating period in 2016, and different concentrations of PM2.5 solutions were prepared by sterile PBS. Healthy ICR pregnant mice were divided into 6 groups randomly: the control group, the PM2.5 low, medium and high dose group, the VC intervention group, and the quercetin intervention group. Mother mice in each group were given solutions for 8 times by intratracheal instillation during pregnancy and lactation; PM2.5 low, medium and high dose groups were given 3.75, 7.5, 15 mg/kg PM2.5 solution respectively, VC and quercetin intervention group were given 15 mg/kg PM2.5 solution, and the control group was given a clean filter elution liquid. Mother mice were administrated by intragastric administration everyday; VC and quercetin intervention group were treated with 100 mg/kg VC or quercetin respectively, and the other groups were treated with solvent. The changes of body weight, food intake of all animals, and growth and neural-developmental indexes of all spring were observed in the whole experimental period.Results The weight of offspring in PND14 in the PM2.5 high dose group was significantly lower than that in the control group (P <0.05, P <0.01). Offspring in the PM2.5 groups had more developmental problems than animals in the control group (P <0.05 and P <0.01) and offspring in the intervention groups had less developmental problems than animals in the PM2.5 high dose group (P <0.05 and P<0.01). Conclusion Certain doses of PM2.5 exposure during pregnancy and lactation affect the development of early physiology and nerve reflex in offspring mice, and the intervention of VC and quercetin can improve the adverse effects to some extent.
张敏佳, 刘伟, 周雅琳, 李雍, 冯金秋, 范爱琴, 许雅君. 孕期和哺乳期暴露PM2.5对子代小鼠生长发育的影响及维生素C和槲皮素的干预研究[J]. 中国生育健康杂志, 2018, 29(1): 25-30.
ZHANG Minjia, LIU Wei, ZHOU Yalin, FENG Jinqiu, FAN Aiqin, LI Yong, XU Yajun. Effects of maternal exposure to PM2.5 during pregnancy and lactation period on growth and development of offspring mice and the protective effects of VC and quercetin. Chinese Journal of Reproductive Health, 2018, 29(1): 25-30.
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