TLR4/NF-κB表达升高促进宫颈癌增殖和转移的作用及机制

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摘要目的研究Toll样受体4(Toll-like Receptor 4,TLR-4)/ 核因子-κB(nuclear factor-kappa B,NF-κB)分子在宫颈癌中的表达,探讨TLR4/ NF-κB表达与宫颈癌增殖、迁移的关系及具体机制。方法采用实时定量PCR(quantitative real-time PCR,qRT-PCR)比较宫颈癌组织及癌旁组织中TLR4/ NF-κB的表达。采用脂质体介导转染方法,根据人宫颈癌细胞株HCE1细胞TLR4表达情况,分为Blank 、阴性对照、TLR4沉默、TLR4过表达4组。采用CCK8、划痕实验、Transwell实验检测TLR4对细胞增殖、迁移、侵袭,并进一步检测Myd88,ERK1/2 及NF-κB信号分子改变。结果肿瘤组织TLR4/ NF-κB的表达显著高于癌旁组织(P＜0.05);宫颈癌细胞株HCE1中TLR4/ NF-κB表达显著高于正常宫颈上皮细胞(P＜0.05)。TLR4沉默组细胞增殖能力较NC组显著下降(P＜0.05),过表达TLR4细胞增殖能力显著升高(P＜0.05)。TLR4沉默组愈合面积比例显著低于NC组(P＜0.05),过表达TLR4组愈合面积比例显著升高(P＜0.05)。TLR4沉默组穿膜细胞数明显少于NC组(P＜0.05),过表达TLR4组穿膜细胞数明显多于NC组(P＜0.05)。TLR4沉默组Myd88,ERK1/2及NF-κB的表达显著低于NC组;TLR4过表达组Myd88,ERK1/2及NF-κB的蛋白表达显著高于NC组(P均＜0.05)。结论 TLR4/NF-κB在宫颈癌中表达升高,促进宫颈癌细胞增殖、迁移、侵袭。

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	蔡静
	张丹
	张燕

关键词 ： TLR4, NF-κB, 宫颈癌, 侵袭, 迁移, 增殖, 病理分级

Abstract：Objective To study the expression of Toll-like Receptor 4 (TLR4) / nuclear factor-kappa B (NF-κB) in cervical cancer, and to explore the relationship between TLR4/ NF-κB expression with proliferation and migration of cervical cancer and its mechanism.Methods Real time quantitative PCR was used to compare the expression of TLR4/ NF-κB in cervical cancer tissues and adjacent tissues. The expression of TLR4 in human cervical cancer cell line HCE1 was silenced or over-expressed by liposome-mediated transfection. The effect of TLR4 on cell proliferation, migration, invasiveness ability was detected by CCK8, scratch test and Transwell assay. The changes of Myd88, ERK1/2 and NF-κB signal molecules were further detected.Results The expression of TLR4/ NF-κB in tumor tissue was significantly higher than that of adjacent tissue (P＜0.05), and the relative expression of TLR4/ NF-κB in HCE1 was significantly higher than that of normal cervical epithelial cells (P＜0.05). The cell proliferation ability of TLR4 silencing group was significantly lower than that of NC group (P＜0.05), while the ability in TLR4 overexpressed cells was significantly higher (P＜0.05). The proportion of healing area in TLR4 silencing group was significantly lower than that in NC group (P＜0.05), while the proportion in TLR4 overexpressed group was significantly increased (P＜0.05). The number of penetrating cells in TLR4 silencing group was significantly less than that in group NC (P＜0.05), while the number in TLR4 overexpressed group was significantly higher (P＜0.05). The expression of Myd88, ERK1/2 and NF-κB in TLR4 silencing group was significantly lower than that in NC group, while their expression in TLR4 overexpressed group was significantly higher (P＜0.05).Conclusion Upregulated TLR4/ NF-κB activation could promote the proliferation, migration and invasion of cervical cancer.

Key words： TLR4 NF-κB cervical cancer invasion migration proliferation pathological grading

收稿日期: 2018-06-05

基金资助:湖北省卫生计生委重点支撑项目 (WJ2017Z002)

通讯作者: 张燕(zyfck@sina.com)

引用本文:

蔡静,张丹,张燕. TLR4/NF-κB表达升高促进宫颈癌增殖和转移的作用及机制[J]. 中国生育健康杂志, 2019, 30(1): 21-25.
CAI Jing, ZHANG Dan, ZHANG Yan. Effect and mechanism of TLR4/ NF-κB signaling pathway on proliferation and metastasis of cervical cancer. Chinese Journal of Reproductive Health, 2019, 30(1): 21-25.

链接本文:

http://cjrh.bjmu.edu.cn/CN/ 或 http://cjrh.bjmu.edu.cn/CN/Y2019/V30/I1/21

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