胆碱能抗炎通路在子痫前期患者母胎界面的表达及意义

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摘要目的探讨胆碱能抗炎通路在子痫前期患者母胎界面的表达及意义。方法 2015年1月—2016年1月本院正常妊娠孕妇、轻度子痫前期患者和重度子痫前期患者各30例。采用qRT-PCR法和Western-blot法分别测定胎盘组织 α7 烟碱型乙酰胆碱受体(alpha-7 nicotinic acetylcholine receptor,α7nAChR) 的mRNA和蛋白表达,免疫组化法检测胎盘组织核因子-κB(nuclear factor-kB,NF-κB)蛋白表达,ELISA法测定胎盘组织白介素6(interleukin 6,IL-6)和肿瘤坏死因子(tumor necrosis factor α,TNF-α)蛋白水平。结果 ①轻度子痫前期组胎盘组织中α7nAChR mRNA的表达水平明显高于对照组和重度组(P<0.01),而重度组明显低于对照组,差异有统计学意义(P<0.05)。α7nAChR蛋白表达结果与mRNA结果基本一致。②子痫前期组胎盘NF-κB蛋白表达均较对照组显著增强,差异有统计学意义(P<0.05),且重度组显著高于轻度组(P<0.05)。③子痫前期组胎盘IL-6和TNF-α蛋白表达明显高于对照组,差异有统计学意义(P<0.05),且重度组显著高于轻度组(P<0.05)。结论胎盘组织中α7nAChR和NF-κB表达异常及炎症介质的增多可能与子痫前期发病有关,胆碱能抗炎通路可能参与子痫前期的发生、发展的病理生理过程。

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	郑林媚
	施蕾
	周忠义
	陈小菊
	王莉
	唐荣
	卢志诚

关键词 ：子痫前期, 胆碱能抗炎通路, α7 烟碱型乙酰胆碱受体, 核因子-κB

Abstract：Objective To investigate the expression and its significance of cholinergic anti-inflammatory pathway in placenta of patients with preeclampsia.Methods mRNA expression and protein of alpha-7 nicotinic acetylcholine receptor (α7nAChR) in placenta was detected by qRT-PCR and Western Blot among 60 women with preeclampsia (including 30 cases of mild preeclampsia and 30 cases of severe preeclampsia) and 30 healthy pregnant women, respectively. Immunohistochemistry was performed to determine the level of activation of nuclear factor-kB (NF-κB) in placentas. ELISA was used to determine cytokine levels, including interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α).Results ①The expression of α7nAChR mRNA in placenta was increased significantly with preeclampsia compare to control group and mild preeclampsia group (P<0.01), it was lower in severe preeclampsia than that in control group (P<0.05). Results of Western blotting assay were consistent with these of qRT-PCR. ② The expression of NF-κB protein in placenta was decreased significantly with preeclampsia compare to control group (P<0.05), and there was a significant increase of NF-κB protein expression in severe preeclampsia group when compare to mild group in placenta (P<0.05). ③ The expression of IL-6 and TNF-α proteins in placenta were increased significantly among preeclampsia patients compare to control group (P<0.05). There were higher in patients with severe preeclampsia than those with mild preeclampsia (P<0.05).Conclusion It suggests that abnormal expression of α7nAChR and NF-κB and excessive pro-inflammatory cytokines in placenta may be related to preeclampsia. Cholinergic anti-inflammatory pathway may play an important role in the pathogenesis of preeclampsia.

Key words： Preeclampsia cholinergic anti-inflammatory pathway α7nAChR NF-κB

收稿日期: 2018-05-10

基金资助:海南省自然科学基金项目(810327)

通讯作者: 施蕾(13198907396@163.com)

引用本文:

郑林媚,施蕾,周忠义,陈小菊,王莉,唐荣,卢志诚. 胆碱能抗炎通路在子痫前期患者母胎界面的表达及意义[J]. 中国生育健康杂志, 2019, 30(1): 15-20.
ZHENG Linmei, SHI Lei, ZHOU Zhongyi, CHEN Xiaoju, WANG Li, TANG Rong, LU Zhicheng. Expression of cholinergic anti-inflammatory pathway in Placenta among Preeclampsia patients. Chinese Journal of Reproductive Health, 2019, 30(1): 15-20.

链接本文:

http://cjrh.bjmu.edu.cn/CN/ 或 http://cjrh.bjmu.edu.cn/CN/Y2019/V30/I1/15

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