Abstract：Objective To investigate whether the human ovarian granulosa cells aging obviously as age increasing . Methods Granulosa cells was collected during the process of picking up oocytes. Real-time PCR method was used to detect the expression of Sirt1, and T/S Ratio method to detect the length of telomere and the proliferation ability of granulosa cells in vitro. Results The expression of Sirt1 of ovarian granulosa cells was not significantly increased, and the telomere length did not significantly shorten, as age increasing. The proliferation ability of granulosa cells maintained high till the age of 45 years. Conclusion The ovarian granulosa cells were not aging significantly, although the eggs was aging obviously as age increasing.
1 Stolwijk,AM,Zielhuis GA,Sauer MV,et al.The impact of the woman′s age on the success of standard and donor in vitro fertilization.Fertil Steril,1997. 67:702-710. 2 Levran D,Ben-Shlomo I,Dor J,et al.Aging of endometrium and oocytes:observations on conception and abortion rates in an egg donation model.Fertil Steril,1991,56:1091-1094. 3 Hook EB.Maternal age,paternal age,and human chromosome abnormality:nature,magnitude,etiology,and mechanisms of effects.Basic Life Sci,1985,36:117-1132. 4 Thiel M,Blanke P,Langler A.Clinical courses of trisomy 18 (Edwards syndrome) - an update.Z Geburtshilfe Neonatol,2011,215:109-114. 5 Pan H,Ma P,Zhu W,et al.Age-associated increase in aneuploidy and changes in gene expression in mouse eggs.Dev Biol,2008,316:397-407. 6 Keefe DL,Marquard K,Liu L.The telomere theory of reproductive senescence in women.Curr Opin Obstet Gynecol,2006,18:280-285. 7 Thomas FH,Vanderhyden BC.Oocyte-granulosa cell interactions during mouse follicular development:regulation of kit ligand expression and its role in oocyte growth.Reprod Biol Endocrinol,2006,4:19. 8 Komatsu K.Masubuchi S.Mouse oocytes connect with granulosa cells by fusing with cell membranes and form a large complex during follicle development.Biol Reprod,2018,99:527-535. 9 Herranz D,Munoz-Martin M,Canamero M.et al.Sirt1 improves healthy ageing and protects from metabolic syndrome-associated cancer.Nat Commun,2010,1:3. 10 张梦,崔华.SIRT1、SIRT1SNP与年龄相关性疾病研究进展.中华保健医学杂志,2016,2:173-176. 11 Lee HW,Blasco MA,Gottlieb GJ,et al.Essential role of mouse telomerase in highly proliferative organs.Nature,1998,392:569-574. 12 Liu L,Trimarchi JR,Navarro P,et al.Oxidative stress contributes to arsenic-induced telomere attrition,chromosome instability,and apoptosis.J Biol Chem,2003,278:31998-32004. 13 Yamanaka S,Takahashi K.Induction of pluripotent stem cells from mouse fibroblast cultures.Tanpakushitsu Kakusan Koso,2006,51:2346-2351. 14 Mao J,Liu L.Generation of iPS Cells from Granulosa Cells.Methods Mol Biol,2016,1357:451-64. 15 Varras M,Griva T,Kalles V,et al.Markers of stem cells in human ovarian granulosa cells:is there a clinical significance in ART? J Ovarian Res,2012,5:36. 16 Bagheripour N,Zavareh S,Ghorbanian MT,et al.Changes in the expression of OCT4 in mouse ovary during estrous cycle.Vet Res Forum,2017,8:43-48. 17 Regan SLP,Knight PG,Yovich JL,et al.Granulosa cell apoptosis in the ovarian follicle-A changing view.Front Endocrinol (Lausanne),2018,9:61. 18 刘梦圆.卵巢衰老.国际生殖健康/计划生育杂志,2014,5:388-391. 19 Nandi S,Tripathi SK,Gupta PSP,et al.Nutritional and metabolic stressors on ovine oocyte development and granulosa cell functions in vitro.Cell Stress Chaperones,2018,23:357-371. 20 张秋芳,刘平.女性生殖衰老及其对人工助孕结局的影响.生殖与避孕,2005:491-494.
