CPEB4沉默对宫颈癌Siha细胞的增殖、迁移和侵袭的影响

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摘要目的探讨胞质聚腺苷酸化物结合蛋白4 (CPEB4) 在宫颈癌中的表达及其对宫颈癌Siha细胞增殖、迁移和侵袭的影响。方法采集本院2017年5月—2018年5月42例宫颈癌患者手术切除的癌变组织及其正常癌旁组织,采用实时定量PCR和Western blot方法检测宫颈癌及其癌旁组织中CPEB4的表达;采用siRNA转染沉默宫颈癌Siha细胞中CPEB4的表达,CCK-8法检测细胞增殖,Transwell检测细胞迁移和侵袭,Western blot检测P53、Twist1的蛋白表达。结果与癌旁组织相比,宫颈癌组织中CPEB4 mRNA和蛋白表达均显著上调;转染CPEB4 siRNA的Siha细胞增殖活性减弱,迁移和侵袭能力均显著下降(P<0.05),且细胞中P53蛋白表达上调、Twist1蛋白表达显著下调(P<0.05)。结论 CPEB4在宫颈癌中高表达,可能通过调控P53和Twist1的表达影响宫颈癌细胞的增殖、迁移与侵袭,有望成为宫颈癌诊断标志物及潜在的治疗靶点。

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	綦春蕾
	于月成

关键词 ： CPEB4, 宫颈癌, 增殖, 迁移, 侵袭

Abstract：Objective To investigate the expression of cytoplasmic polyadenylation element binding protein 4 (CPEB4) in cervical cancer and its effect on proliferation, migration and invasion of cervical cancer Siha cells.Methods We collected cancerous tissues and normal adjacent tissues of 42 patients with cervical cancer. Quantitative real-time PCR and Western blot were performed to detect mRNA and protein levels of CPEB4 in cervical cancer and pericarcinomatous tissues. CKK-8 assay, transwell assay and western blot were used to evaluate cell proliferation, migration and invasion, and protein expression of P53 and Twist1 after transfection of CPEB4 siRNA in cervical cancer Siha cells.Results Compared with pericarcinomatous tissues, the mRNA and protein expression of CPEB4 were both significantly up-regulated in cervical cancer tissues. The proliferation activity of Siha cells transfected with CPEB4 siRNA was weakened, and the migration and invasion ability were significantly decreased (P<0.05). Moreover, the protein expression of P53 was significantly up-regulated and Twist1 was significantly down-regulated in Siha cells transfected with CPEB4 siRNA (P<0.05).Conclusion The high expression of CPEB4 in cervical cancer may affect the proliferation, migration and invasion of cervical cancer cells by regulating the expression of P53 and Twist1, and CPEB4 may be a diagnostic marker and potential therapeutic target for cervical cancer.

Key words： cytoplasmic polyadenylation element binding protein 4 cervical cancer proliferation migration invasion

收稿日期: 2019-01-30

通讯作者: 于月成(yuechengyxa@163.com)

引用本文:

綦春蕾, 于月成. CPEB4沉默对宫颈癌Siha细胞的增殖、迁移和侵袭的影响[J]. 中国生育健康杂志, 2019, 30(6): 530-533.
QI Chunlei, YU Yuecheng. Effect of CPEB4 silencing on proliferation, migration and invasion of cervical cancer Siha cells. Chinese Journal of Reproductive Health, 2019, 30(6): 530-533.

链接本文:

http://cjrh.bjmu.edu.cn/CN/ 或 http://cjrh.bjmu.edu.cn/CN/Y2019/V30/I6/530

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