Optimization of sampling method can improve the efficiency of noninvasive preimplantation genetic testing for aneuploidy
HUANG Jin, JIA Jialin, DANG Yujiao, QIAO Jie, LIU Ping
Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital; National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital); Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, China
Abstract：Objective To explore the feasibility of noninvasive preimplantation genetic testing for aneuploidy (NiPGT-A) and the optimization method of sample retention. Methods 239 PGT-A blastocysts from Center for Reproductive Medicine of Peking University Third Hospital were included. The PGA-A indications of included women were older age (>38 years), recurrent abortion, and repeated implantation failure. If the blastocyst was up to the biopsy standard, the corresponding culture medium was collected. The collection method was divided into three stages according to embryo drilling and the culture duration. The trophectoderm cells were detected by SNP array, while NiPGT-A was detected by next generation sequencing(NGS). PGT-A and NiPGT-A results including the detection rate of NiPGT-A, the consistency of NiPGT-A and PGT-A results, the coincidence rate of aneuploidy and granulosa cell pollution rate, were compared back-to-back. Only 56 PGT-A euploid embryos were transferred and the NiPGT-A results of the transferred blastocysts were analyzed. Results Among 239 blastocysts, 209 blastocysts had NiPGT-A results, and the detection rate of NiPGT-A was 87.5%. With the improvement of sampling method, the consistency of ploidy and aneuploidy of NiPGT-A results and PGT-A results ewere gradually increased; the pollution rate of granular cells was gradually reduced. Among 56 PGT-A euploidy blastocysts transferred, 23 blastocysts got live births or ongoing pregnancies. However, the clinical pregnancy rate was 71.4% in the group of the embryos without NiPGT-A result, higher than that of the group of NiPGT-A euploidy (33.3%) and the group of NiPGT-A aneuploidy (40.9%). Conclusion It is recommended to collect blastocyst culture medium from D4 to D5/D6, without drilling the embryos. With continuous improvement of NiPGT-A technology, it is expected to be used in clinical practice.
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