The levels of 8-OHdG and 4-HNE in follicular fluid among patients with decreased ovarian reserve function and their relationship with in vitro fertilization-related indexes
LIANG Chengcheng, ZHANG Xiaole, QI Cong, HU Hui, ZHANG Qinhua, ZHU Xiuxian, FU Yonglun, GU Yuting, SHEN Cong
Department of Gynecology,Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Abstract：Objective To investigate the levels of 8-hydroxydeoxyguanosine (8-OHdG) and 4-hydroxynonenal (4-HNE) in follicular fluid among patients with decreased ovarian reserve function (DOR) and their relationship with related indexes of in vitro fertilization (IVF).Methods The follicular fluid of patients undergoing IVF-assisted pregnancy in the Department of Reproduction Medicine of Shanghai First Maternity and Infant Hospital from July 2020 to July 2021 were collected for analysis. Among them, 37 patients with DOR infertility were included in the DOR group, and 42 women with normal ovarian reserve function (NOR) due to fallopian tube factors were selected as control group. The levels of 8-OHdG, 4-HNE and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) were compared between the two groups, and the relationship between 8-OHdG, 4-HNE and IVF-related indexes was analyzed.Results Compared with the control group, the number of oocytes obtained, the number of MⅡ, the number of fertilization and high-quality embryos in DOR group were significantly decreased (P<0.05). The levels of 8-OHdG and 4-HNE in follicular fluid of patients with DOR group were significantly increased, while the activities of SOD, GSH-Px and CAT were significantly decreased (P<0.05). Correlation analysis results showed that the levels of 8-OHdG and 4-HNE in follicular fluid of patients with DOR group were positively correlated with age and basal FSH (r>0, P<0.05), while 8-OHdG was negatively correlated with AMH, number of fertilization and high-quality embryos (r<0, P<0.05). 4-HNE was negatively correlated with AMH, MⅡ number and number of fertilization (r<0, P<0.05).Conclusion Oxidative stress reaction exists in follicular fluid of DOR patients, which may affect the maturation, fertilization and embryonic potiential development, and then affect the outcome of IVF pregnancy in DOR patients.
梁程程, 章晓乐, 齐聪, 胡慧, 张勤华, 朱秀娴, 傅永伦, 顾雨婷, 沈聪. 卵巢储备功能下降患者卵泡液中8-OHdG和4-HNE的水平及其与体外受精相关指标的关系[J]. 中国生育健康杂志, 2022, 33(6): 525-531.
LIANG Chengcheng, ZHANG Xiaole, QI Cong, HU Hui, ZHANG Qinhua, ZHU Xiuxian, FU Yonglun, GU Yuting, SHEN Cong. The levels of 8-OHdG and 4-HNE in follicular fluid among patients with decreased ovarian reserve function and their relationship with in vitro fertilization-related indexes. Chinese Journal of Reproductive Health, 2022, 33(6): 525-531.
