The relationship between the expression of LncRNA HIF1A-AS1 and HIF1A in placental tissues with the recasting of placental spiral artery in late onset severe preeclampsia
ZHANG Jing, XU Lin, YE Yuanhua
Qingdao University School of Medicine, Qingdao 266000, Shandong
Abstract：Objective To analyze the relationship between the abnormal expression of long non-coding RNA hypoxia-inducible factor 1 alpha-antisense RNA 1 (LncRNA HIF1A-AS1) and hypoxia-inducible factor-1 alpha (HIF1A) in placental tissues with the placental spiral artery recasting of pregnant women with late onset severe preeclampsia. Methods We selected ninety-two pregnant women with late onset severe preeclampsia who gave birth in our hospital from October 2019 to April 2021 as the preeclampsia group, and 86 pregnant women with normal pregnancy during the same period as the normal pregnancy group. We also collected the general data about age, systolic blood pressure and diastolic blood pressure of each pregnant woman. Real-time fluorescent quantitative PCR (qRT-PCR) method was used to detect the levels of LncRNA HIF1A-AS1 and HIF1A mRNA in the placental tissue, and scanning electron microscope was used to measure the lumen area and wall thickness of the placental spiral artery. We used Pearson′s method to analyze the correlation between LncRNA HIF1A-AS1 and HIF1A mRNA levels with different parameters in the placental tissues of pregnant women with late onset severe preeclampsia. Results Compared with the normal pregnancy group, the level of HIF1A mRNA in the placental tissue, average wall thickness, systolic blood pressure and diastolic blood pressure were significantly increased in the preeclampsia group. The LncRNA HIF1A-AS1 level and the average lumen area were significantly decreased (P<0.05). There was a negative correlation between LncRNA HIF1A-AS1 and HIF1A mRNA levels in the placental tissue of pregnant women with late onset severe preeclampsia (r=-0.628, P<0.05). LncRNA HIF1A-AS1 was positively correlated with the average lumen area (P<0.05), and was negatively correlated with the average tube wall thickness, systolic blood pressure and diastolic blood pressure (P<0.05). HIF1A mRNA level was negatively correlated with the average lumen area (P<0.05), and positively correlated with the average tube wall thickness, systolic blood pressure and diastolic blood pressure (P<0.05). Conclusion The expression of LncRNA HIF1A-AS1 and HIF1A is abnormal in the placental tissue of pregnant women with late onset severe preeclampsia. It may be related to insufficient recasting of the placental spiral artery.
张静, 徐琳, 叶元华. 晚发型重度子痫前期胎盘组织LncRNA HIF1A-AS1、HIF1A表达与胎盘螺旋动脉重铸的关系[J]. 中国生育健康杂志, 2023, 34(1): 51-55.
ZHANG Jing, XU Lin, YE Yuanhua. The relationship between the expression of LncRNA HIF1A-AS1 and HIF1A in placental tissues with the recasting of placental spiral artery in late onset severe preeclampsia. Chinese Journal of Reproductive Health, 2023, 34(1): 51-55.
1 杨怡珂,漆洪波.美国妇产科医师学会(ACOG)“妊娠期高血压和子痫前期指南2019版”要点解读(第一部分).中国实用妇科与产科杂志,2019,35:895-899. 2 Yang Y,Li H,Ma Y,et al.MiR-221-3p is down-regulated in preeclampsia and affects trophoblast growth,invasion and migration partly via targeting thrombospondin 2. Biomed Pharmacother,2019,109:127-134. 3 张翠翠,谢玲,李洁.重度子痫前期患者血清和胎盘VEGF、sVEGFR-1水平异常与lncRNA-LOC391533表达的相关性分析.临床和实验医学杂志,2019,18:2293-2296. 4 Xue X,Luo L.LncRNA HIF1A-AS1 contributes to ventricular remodeling after myocardial ischemia/reperfusion injury by adsorption of microRNA-204 to regulating SOCS2 expression.Cell Cycle,2019,18:2465-2480. 5 Hong F,Gao Y,Li Y,et al.Inhibition of HIF1A-AS1 promoted starvation-induced hepatocellular carcinoma cell apoptosis by reducing HIF-1α/mTOR-mediated autophagy.World J Surg Oncol,2020,18:113. 6 李山,蒋向阳,刘顺达.低氧诱导因子-1α、血管内皮生长因子、Bcl-2与急性脑梗死患者神经功能缺损度的相关研究.神经损伤与功能重建,2019,14:79-82. 7 谢幸,孔北华,段涛.妇产科学.9版.北京:人民卫生出版,2018:105-109. 8 Sebastian A,Raj T,Yenuberi H,et al.Angiogenic factors and uterine artery Doppler in predicting preeclampsia and associated adverse outcomes in a tertiary hospital in south India.Pregnancy Hypertens,2019,16:26-30. 9 Wang L,Zhang Y,Qu H,et al.Reduced ELABELA expression attenuates trophoblast invasion through the PI3K/AKT/mTOR pathway in early onset preeclampsia.Placenta,2019,87:38-45. 10 Zhang Y,He XY,Qin S,et al.Upregulation of PUM1 expression in preeclampsia impairs trophoblast invasion by negatively regulating the expression of the lncRNA HOTAIR.Mol Ther,2020,28:631-641. 11 Allerkamp HH,Clark AR,Lee TC,et al.Something old,something new:digital quantification of uterine vascular remodelling and trophoblast plugging in historical collections provides new insight into adaptation of the utero-placental circulation.Hum Reprod,2021,36:571-586. 12 徐欣然,王妍平,崔洪艳.胎盘外泌体在子宫螺旋动脉重铸不全相关妊娠并发症中的作用.国际妇产科学杂志,2019,46:288-292. 13 Kosovic I,Prusac IK,Mestrovic Z,et al.HIF-1α immunohistochemical expression in decidual cells,villous and extravillous trophoblast in placentas from pregnancies complicated with preeclampsia.Pregnancy Hypertens,2020,21:176-178. 14 Vetrovoy OV,Nimiritsky PP,Tyulkova EI,et al.The content and activity of hypoxia-inducible factor HIF1α increased in the hippocampus of newborn rats that were subjected to prenatal hypoxia on days 14-16 of embryogenesis.Neurochem J,2020,14:286-289. 15 徐峰,王莹,鲁晓燕,等.HIF-1α、TLR4与稽留流产妊娠滋养细胞凋亡的关系研究.中国计划生育和妇产科,2020,12:73-77. 16 Albers RE,Kaufman MR,Natale BV,et al.Trophoblast-Specific Expression of Hif-1α Results in Preeclampsia-Like Symptoms and Fetal Growth Restriction.Sci Rep,2019,9:2742. 17 Wu D,Yang N,Xu Y,et al.lncRNA HIF1A antisense RNA 2 modulates trophoblast cell invasion and proliferation through upregulating PHLDA1 expression.Mol Ther Nucleic Acids,2019,16:605-615. 18 何美荣,王彩珊.缺氧诱导因子HIF-1a在子痫前期患者胎盘中的表达.中国保健营养,2019,29:292.
