Abstract:Objective To characterize the expression of internal protein (endoglin), vascular endothelial growth factor (VEGF) and its receptor 1 (Flt-1) in serum and placenta tissue of patients with preeclampsia, and to examine the clinical implications. Methods Fifteen cases of patients with mild preeclampsia, 30 cases of patients with severe preeclampsia and 20 cases of normal term pregnant women (control group) were selected. Concentrations of endoglin, VEGF, and Flt-1 in serum were determined by ELISA kit. At delivery, placental samples were collected, and the expression and distribution of endoglin, VEGF, and Flt-1 were determined by immunohistochemistry. Results Endoglin level in serum of the control, mild, and severe preeclampsia group was (0.9±0.1) ng/ml, (1.1±0.2) ng/ml, (1.4±0.3) ng/ml, respectively. Serum Flt-1 concentration in the control, mild and severe preeclampsia group was (204.0±33.4) ng/L, (562.0±60.7) ng/L, (756.0±71.3) ng/L, respectively. Serum VEGF concentration in the control, mild, and severe preeclampsia group was (151.0±13.1) ng/L, (81.4±6.3)] ng/L, (63.2±5.2) ng/L, respectively. Positive expression rate of endoglin in placenta tissue of the control, mild, and severe preeclampsia was 36.0%, 61.3%, and 79.3%, respectively, with expression quantities of (86.72±6.35),(97.46±6.91), and (113.57±7.04), respectively. The positive expression rate of Flt-1 was 48.0%, 70.7%, and 84.0%, respectively, with expression quantities of (126.12±7.80), (141.86±7.17), and (154.32±8.01), respectively. The positive expression rate of VEGF in the control, mild, and severe preeclampsia placenta tissue was 57.0%, 86.7%, and 91.3%, respectively, with expression quantities of 133.50±7.14, 117.28±6.46, and 103.69±6.84, respectively. In placenta tissue, the expression of endoglin, VEGF, and Flt-1 was not correlated; the expression of VEGF and Flt-1 was negatively correlated. Conclusion Endoglin, VEGF, and Flt-1 were closely related to the occurrence and progression of preeclampsia, and therefore may be used as auxiliary indicator to evaluate the clinical diagnosis and prognosis.
孙颖, 余艳红, 周紫琼, 李智泉. 内皮糖蛋白、血管内皮生长因子及其受体1在子痫前期中的表达和临床意义[J]. 中国生育健康杂志, 2015, 26(2): 123-127.
SUN Ying, YU Yanhong, ZHOU Ziqiong, LI Zhiquan. Expression and clinical implication of endothelial glycoprotein, vascular endothelial growth factor and its receptor 1 in preeclampsia. Chinese Journal of Reproductive Health, 2015, 26(2): 123-127.
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