Abstract：Objective To establish the method of extraction, purification and identification of folate receptor from human Placenta.Methods A placenta was collected from a healthy woman when delivering in a hospital. The placental tissue was homogenized after cutting. Target protein was extracted by affinity chromatography from the homogenate after acidification and neutralization, then was purified by chemical and physical methods. The protein extraction was quantified by Bradford protein assay. The molecular weight was determined through sodium dodecyl sulfate polyacrylamide gel electrophoresis and silver stain. Identification of the protein was performed by Western Blot.Results The wet weight of the placenta used for protein extraction was 370 g. Target protein was eluted into fraction2-5 among 8 fractions after extraction by affinity chromatography and was further purified. Results of Bradford protein assay showed that a total of 345 μg protein was obtained. The molecular weight was about 35-40 KDa. Western Blot showed that the protein could specifically bind to the antibody to folate receptor.Conclusion The method of extraction, purification and identification of folate receptor from human placenta was successfully established. 93 μg of folate receptor could be obtained from placenta per 100 g. The method in this study provides a good foundation for the detection of antibody to folate receptor.
王琳琳,袁悦,任爱国. 人胎盘叶酸受体蛋白的提取、纯化及鉴定[J]. 中国生育健康杂志, 2014, 25(1): 37-40.
WANG Linlin, YUAN Yue, REN Aiguo. Extraction, purification and identification of folate receptor from human placenta. Chinese Journal of Reproductive Health, 2014, 25(1): 37-40.
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