Abstract：Objective To investigate the effect of controlled hyperstimulation on placental barrier. Methods A total of 121 pregnant women who met the criteria from January 2014 to December 2014 were selected. Among them, 79 cases were in artificial pregnancy group (39 cases in fresh embryo group and 40 cases in freeze-thaw group), 42 cases in normal pregnancy group were in natural pregnancy. The quantitative analysis of the placental villi tissue and the microstructural observation of the placenta were carried out. Results Compared with the normal pregnancy group, the number of villus vessels in the artificial pregnancy group (fresh embryo group and freeze-thaw group) decreased, and the ratio of blood vessels to the cross section area of villus decreased (P<0.05), but there was no difference in villus density (P>0.05). There was no significant difference in the above indexes between fresh embryo group and freeze-thaw group. The changes of vesicle-like and calcification in the ST cytoplasm of placenta from fresh embryo group and freeze-thaw group were more prone than that of normal pregnancy group. There was no significant difference in residual nucleus pyknosis, calcification, villus interstitial edema and cellulose deposition between fresh embryo group and freeze-thaw group. Conclusion There was no significant change in histology of hematoxylin-eosin staining in fresh embryo group and freeze-thaw group, and the basic structure of ultrastructure display barrier was normal. Therefore, there is no evidence that the controlled ovarian hyperstimulation cycle is the cause of the above changes in placenta in the assisted pregnancy group, and some ultrastructural differences in placenta need to be further studied.
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