卵裂期胚胎可溶性人类白细胞抗原G与妊娠结局的关系

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摘要目的探讨卵裂期胚胎培养液中可溶性人类白细胞抗原G(sHLA-G)表达与胚胎发育和妊娠结局的关系。方法选择2017年5月—2018年5月在江苏省苏北人民医院生殖医学中心接受冻融胚胎移植的86例不孕患者,共计100个周期为研究对象。根据妊娠结局分为妊娠组和未妊娠组,采取回顾性分析方法,比较两组基本信息及sHLA-G含量差异;再根据sHLA-G的测定结果分为sHLA-G阳性组及阴性组,比较两组的植入率。采用酶联免疫吸附实验(ELISA)检测卵裂期胚胎(体外培养第三天)培养液中sHLA-G浓度。结果妊娠组sHLA-G平均值高于非妊娠组,组间差异有统计学意义(P＜0.05);sHLA-G阳性组的sHLA-G含量平均值及植入率均大于sHLA-G阴性组,差异有统计学意义(P<0.05);二元Logistic回归分析结果显示,胚胎培养液中sHLA-G含量为影响胚胎植入的独立危险因素(P＜0.05)。结论第三天胚胎培养液中sHLA-G 的表达及表达量与胚胎植入有关,可作为妊娠结局的预测指标。

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	耿蒙慧
	邢阿英
	王大琳
	胡艳秋

关键词 ：辅助生殖, 可溶性人类白细胞抗原G, 胚胎植入, 胚胎质量

Abstract：Objective To investigate the relationship between the expression of soluble human leukocyte antigen G (sHLA-G) in cleavage medium of embryo and embryonic development and pregnancy obtained. Methods 86 patients of infertility who received Frozen-thawed embryo transfer at the Reproductive Medical Center of Northern Jiangsu People′s Hospital from May 2017 to May 2018 were selected, a total of 100 cycles were included in the study. According to the pregnancy outcomes, pregnant group and non-pregnant group were divided. The basic clinical data and difference of sHLA-G content between the two groups were compared by a retrospective analysis. Furthermore, the patients were divided into sHLA-G positive and negative group according to the result of sHLA-G measurement and the implantation rate was compared between the two groups. Enzyme-linked immunosorbent assay (ELISA) was used to detect the concentration of sHLA-G in the culture medium of cleavage. Results The average sHLA-G concentration was significantly higher in pregnant group than in non-pregnant group (P<0.05).The average sHLA-G concentration and the implantation rate of sHLA-G positive group were significant higher than sHLA-G negative group (P<0.05).The results of binary logistic regression analysis showed that the content of sHLA-G in embryonic culture medium was an independent risk factor for embryo implantation. Conclusion The expression of sHLA-G in D3 medium of embryo was associated with embryo implantation, which can be used as a potential marker of pregnancyoutcome

Key words： Assisted reproduction Soluble leukocyte antigen G embryo implantation embryo quality

收稿日期: 2018-11-12

基金资助:2018年省级重点研发计划专项基金(BE2018685);生殖医学国家重点实验室开放基金(SKLRM-K201905)

通讯作者: 胡艳秋(huyanqiu78@163.com)

引用本文:

耿蒙慧, 邢阿英, 王大琳, 胡艳秋. 卵裂期胚胎可溶性人类白细胞抗原G与妊娠结局的关系[J]. 中国生育健康杂志, 2021, 32(3): 233-237. GENG Menghui, XING Aying, WANG Dalin, HU Yanqiu. Association on Soluble Human Leukocyte Antigen G and Pregnancy Outcomes in Cleavage Embryos. Chinese Journal of Reproductive Health, 2021, 32(3): 233-237.

链接本文:

http://cjrh.bjmu.edu.cn/CN/ 或 http://cjrh.bjmu.edu.cn/CN/Y2021/V32/I3/233

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