目的 通过比较新疆维吾尔族妇女宫颈癌组织与正常宫颈中NF-κB表达水平的差异,了解新疆维吾尔族妇女宫颈癌组织与NF-κB的关系及意义。方法 采用含 20 000 条 Oligo DNA 的人类全基因组寡核苷酸芯片,对5 例新疆维吾尔族妇女宫颈癌组织与5 例正常宫颈成组检测差异表达基因,通过 MAS 分析得到两组间差异表达基因NF-κB。采用实时荧光定量PCR及免疫组化对62例宫颈癌,17例正常宫颈组织中NF-κB进行检测,并分析各分子表达水平与相应临床病理指标的关系。结果 62例宫颈癌组织NF-κB mRNA的相对表达量为(0.668±0.045),17例正常宫颈NF-κB mRNA的相对表达量为(0.473±0.032),宫颈癌组织中NF-κBmRNA的相对表达量均显著高于正常宫颈组,差异有统计学意义。免疫组织化学SP法结果显示(1)NF-κB蛋白在宫颈癌组和正常宫颈组中阳性表达率分别为51.61% 和11.76%。釆用组间卡方检验,差异有统计学意义。(2)宫颈癌组织中NF-kB蛋白阳性表达与临床和病理资料相关性分析结果显示,年龄≥65岁患者NF-kB蛋白阳性表达率为72.73%,年龄＜65岁患者 NF-κB蛋白阳性表达率为51.72%,卡方检验显示差异无统计学意义;NF-κB蛋白在宫颈癌低分化组、中分化组的表达水平明显均高于高分化组,差异有统计学意义; NF-κB蛋白在宫颈癌III期,IV期的表达水平均显著高于I+II期,差异有统计学意义。结论 NF-κBmRNA和蛋白在宫颈癌中存在高表达,NF-kB蛋白的阳性表达随着宫颈癌的分化程度和临床分期的升高逐步升高。
关键词
宫颈肿瘤 /
全基因组寡核苷酸芯片 /
实时荧光定量PCR /
免疫组化 /
NF-κB
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参考文献
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脚注
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基金
新疆医科大学科研创新基金项目(XYDCX2014)
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