子宫内膜异位症患者血清中miR-199a-5p和基质金属蛋白酶-9的表达及意义

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摘要目的探讨miR-199a-5p和基质金属蛋白酶-9(MMP-9)在子宫内膜异位症(EMS)患者血清中的表达及临床意义。方法分别采用SYBR Green I实时荧光定量PCR方法及ELISA法检测40例EMS患者(EMS组)与20例输卵管积水患者(对照组)血清中miR-199a-5p和MMP-9的表达,分析miR-199a-5p和MMP-9的相关性及其与子宫内膜异位症临床特征的关系。结果两组血清中均检测出miR-199a-5p和MMP-9的表达,EMS组血清中miRNA-199a-5p表达少于对照组,MMP-9含量高于对照组,差异均有统计学意义。EMS组血清miR-199a-5p相对表达量随r-AFS分期增加而减少,MMP-9表达量随r-AFS分期增加而增加,差异均有统计学意义;EMS组血清中miR-199a-5p和MMP-9的表达量与VAS评分无关。EMS组血清中miR-199a-5p相对表达量与MMP-9呈负相关(r=-0.501)。结论 miR-199a-5p和MMP-9可能参与EMS的发病,且发病机制可能与miRNA调控异常相关。

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	汪雁君
	王化丽
	王均荣
	路云晖

关键词 ： miR-199a-5p, 基质金属蛋白酶-9, 子宫内膜异位症

Abstract：Objective To examine the expression of miR-199a-5p and MMP-9 in the serum of patients with endometriosis, and the clinical significance was explored.Methods Using SYBR Green I real-time PCR and ELISA we detected the differential expression of miR-199a-5p and MMP-9 in 40 cases of endometriosis and 20 cases of health controls. We analyzed the correlation between miR-199a-5p and MMP-9 and their clinical implications. Results The expression level of miR-199a-5p was found to be decreased in the endometriosis group compared with the control group (P<0.05), and miRNA-199a-5p was negatively correlated with r-AFS staging of endometriosis (P=0.038), but had no relationship with the degree of dysmenorrhea (P=0.151). The expression level of MMP-9 in the endometriosis group was higher than that in the control group (P<0.01), and MMP-9 increased with r-AFS staging (P=0.028). No relationship with dysmenorrhea degree (P=0.174) was observed. The expression level of miR-199a-5p and MMP-9 was significantly negatively correlated.Conclusion miR-199a-5p and MMP-9 may be involved in the pathogenesis of endometriosis, which may be related to the abnormal regulation of miRNA.

Key words： miR-199a-5p MMP-9 Endometriosis

通讯作者: 王化丽(whl-dl@163.com)

引用本文:

汪雁君, 王化丽, 王均荣, 路云晖. 子宫内膜异位症患者血清中miR-199a-5p和基质金属蛋白酶-9的表达及意义[J]. 中国生育健康杂志, 2016, 27(3): 226-229.
WANGYanjun, WANG Huali, WANG Junrong, LU Yunhui. Expression and implication of miR-199a-5p and MMP-9 in the serum of patients with endometriosis. Chinese Journal of Reproductive Health, 2016, 27(3): 226-229.

链接本文:

http://cjrh.bjmu.edu.cn/CN/ 或 http://cjrh.bjmu.edu.cn/CN/Y2016/V27/I3/226

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