利用胚胎实时监测形态动力学参数预测胚胎种植结局

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摘要目的探索影响胚胎种植结局的胚胎形态动力学参数和胚胎发育特殊分裂模式,以提高体外受精-胚胎移植(IVF-ET)周期的临床结局,缩短治疗周期。方法回顾性分析江苏省苏州市立医院生殖与遗传中心2016年7月到2017年12月212例IVF-ET周期胚胎实时监测(Time-lapse)观察培养的273枚胚胎,其中女方年龄<35岁,143个周期185枚胚胎(其中种值86枚,未种植99枚);女方年龄≥35岁,69个周期88枚胚胎(其中种植30枚,未种植58枚)。探讨胚胎形态动力学指标(t2、t3、t4、t5、t8、cc2、cc3、s2和s3)以及胚胎发育特殊分裂模式(AC、RC和MN)与胚胎种植的相关性。结果在所有273枚胚胎中,种植116枚,未种植157枚,种植组的女方年龄、s2以及AC显著小于未种植组(P均<0.05);对于女方年龄<35岁的患者,种植组<35岁的s3和AC显著低于未种植组<35岁(P<0.05),其余指标组间差异无统计学意义;对于女方年龄≥35岁的患者,种植组≥35岁的s2显著低于未种植组≥35岁(P<0.05),其余指标组间差异无统计学意义。结论胚胎形态动力学参数s2、s3和特殊分裂模式AC与不同年龄患者的胚胎种植潜能具有一定相关性。

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	郑爱燕
	丁洁
	蒲艳
	廖桂芝
	孟庆霞
	王馥新
	李红
	王玮

关键词 ：胚胎实时监测技术, 胚胎, 种植, 形态动力学, 异常分裂

收稿日期: 2019-03-19

基金资助:江苏省妇幼健康科研项目(F201820);江苏省妇幼重点人才(FRC201725);江苏省医学创新团队(CXTDB2017013);苏州市临床医学中心(SZZX201505)

通讯作者: 王玮(ww126626@163.com)

引用本文:

郑爱燕, 丁洁, 蒲艳, 廖桂芝, 孟庆霞, 王馥新, 李红, 王玮. 利用胚胎实时监测形态动力学参数预测胚胎种植结局[J]. 中国生育健康杂志, 2020, 31(2): 161-164.

链接本文:

http://cjrh.bjmu.edu.cn/CN/ 或 http://cjrh.bjmu.edu.cn/CN/Y2020/V31/I2/161

1 Zaninovic N,Irani M,Meseguer M.Assessment of embryo morphology and developmental dynamics by time-lapse microscopy:is there a relation to implantation and ploidy? Fertil Steril,2017,108:722-729.
2 Yang SH,Wu CH,Chen YC,et al.Effect of morphokinetics and morphological dynamics of cleavage stage on embryo developmental potential:A time-lapse study.Taiwan J Obstet Gynecol,2018,57:76-82.
3 Coticchio G,Mignini Renzini M,Novara PV,et al.Focused time-lapse analysis reveals novel aspects of human fertilization and suggests new parameters of embryo viability.Hum Reprod,2018,33:23-31.
4 Kazdar N,Brugnon F,Bouche C,et al.Comparison of human embryomorphokinetic parameters in sequential or global culture media.Ann Biol Clin (Paris),2017,75:403-410.
5 Bodri D,Sugimoto T,Serna JY,et al.Influence of different oocyte insemination techniques on early and late morphokinetic parameters:retrospective analysis of 500 time-lapse monitored blastocysts.Fertil Steril,2015,104:1175-1181.e1-2.
6 Payne D,Flaherty SP,Barry MF,et al.Preliminary observations on polar body extrusion and pronuclear formation in human oocytes using time-lapse video cinematography.Hum Reprod (Oxford),1997,12:532-541.
7 樊玲艳.异常卵裂的胚胎能够产生活产婴儿—一项Time-lapse研究,2016.
8 Meseguer M,Herrero J,Tejera A,et al.The use of morphokinetics as a predictor of embryo implantation.Hum Reprod (Oxford),2011,26:2658-2671.
9 Rubio I,Kuhlmann R,Agerholm I,et al.Limited implantation success of direct-cleaved human zygotes:a time-lapse study.Fertil Steril,2012,98:1458-1463.
10 Goodman LR,Goldberg J,Falcone T,et al.Does the addition of time-lapse morphokinetics in the selection of embryos for transfer improve pregnancy rates?A randomized controlled trial.Fertil Steril,2016,105:275-285.e10.
11 Zhan Q,Ye Z,Clarke R,et al.Direct unequal cleavages:embryo developmental competence,genetic constitution and clinical outcome.PLoS One,2016,11:e0166398.
12 郑爱燕,孟庆霞,丁洁,等.利用胚胎实时观测技术预测早期胚胎的发育潜能.生殖医学杂志,2018,27:38-42.
13 Desai N,Goldberg JM,Austin C,et al.Are cleavage anomalies,multinucleation,or specific cell cycle kinetics observed with time-lapse imaging predictive of embryo developmental capacity or ploidy?Fertil Steril,2018,109:665-674.
14 Liu Y,Chapple V,Roberts P,et al.Prevalence,consequence,and significance of reverse cleavage by human embryos viewed with the use of the Embryoscope time-lapse video system.Fertil Steril,2014,102:1295-300.e2.
15 Aguilar J,Rubio I,Munoz E,et al.Study of nucleation status in the second cell cycle of human embryo and its impact on implantation rate.Fertil Steril,2016,106:291-299.e2.
16 Fragouli E,Alfarawati S,Spath K,et al.The origin and impact of embryonic aneuploidy.Hum Genet,2013,132:1001-1013.
17 Jackson KV,Ginsburg ES,Hornstein MD,et al.Multinucleation in normally fertilized embryos is associated with an accelerated ovulation induction response and lower implantation and pregnancy rates in in vitro fertilization-embryo transfer cycles.Fertil Steril,1998,70:60-66.