Abstract：Objective To investigate the effect of invasion and metastasis ability of cervical cancer cells when their forhead box C1 (FOXC1) gene was knocked down by small interfering RNA (siRNA).Methods The expression of FOXC1 gene in NC104, CaSki, me-180 and HeLa cells of cervical cancer was detected, and the HeLa cell lines with high FOXC1 expression were screened out. The specific lentiviral interference vector foxc1-shrna was constructed to infect cell lines, and the transfection efficiency was detected by Western blot and real-time PCR. The samples were divided into three groups:infection negative group (HeLa), blank control group (hela-gfp), and infection foxc1-shrna group (hela-foxc1 (-)). The migration and invasiveness of the transfected cells were determined by Transwell assay and Matrigel. To further verify the changes of migration and invasion of the three types of cells after transfection, the changes of emt-related proteins such as Vimentin, e-cadherin and β-catenin in the three groups of cells after transfection were detected by Western Blot.Results Real-time PCR and Western blot results indicated that the expression of FOXC1 mRNA and FOXC1 protein were inhibited in the hela-foxc1 (-) group. The Transwell migration experiment showed that the migration ability of cells in the hela-foxc1 (-) group was significantly reduced. The Transwell and Matrigel invasion experiment showed that the invasion ability of cells in the hela-foxc1 (-) group was significantly reduced. Western blot analysis showed that the expressions of Vimentin, e-cadherin and β-catenin in the three groups were significantly decreased. The expression of e-cadherin protein was increased. These differences among the three groups were considered statistically significant (P<0.05).Conclusion We found that knockdown of FOXC1 gene suppressed the expression of compound β-catenin and Vimentin. The increased expression of e-cadherin protein can affect biological behavior of cervical cancer cells and decrease their invasion and metastasis ability.
张婕, 汪露, 韩世愈. 沉默FOXC1对宫颈癌HeLa细胞侵袭和转移能力的影响[J]. 中国生育健康杂志, 2021, 32(6): 540-545.
ZHANG Jie, WANG Lu, HAN Shiyu. Knockdown of FOXC1 in a cervical cancer Hela cell line and its effects on cell invasion and metastasis. Chinese Journal of Reproductive Health, 2021, 32(6): 540-545.
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