子痫前期患者及其胎儿血清、胎盘组织中趋化因子的表达水平变化及意义

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摘要目的探究趋化因子在子痫前期中的变化和意义。方法选取在本院妇产科住院诊治及分娩的30名子痫前期孕妇作为研究组,同期30名健康孕妇作为对照组。采集两组孕妇外周血和新生儿脐带血,收集胎盘组织,利用试剂盒检测孕妇血清肌酐(Cr)、血尿素氮(BUN)和C-反应蛋白(CRP),应用ELISA检测孕妇和新生儿血清CXCL9、CXCL10、CXCL12表达水平,应用Western blot检测胎盘组织CXCL9、CXCL10、CXCL12表达水平。结果与正常组健康孕妇相比,研究组子痫前期孕妇的平均分娩孕周 [(36.5±1.0)周]短,BMI [(30.1±0.5)kg/m²] 高,剖宫产(69.0%)、新生儿体重不足2.5 kg(60.0%)以及胎儿生长受限(36.7%)的几率都显著性升高(P<0.05)。肾功能相关生化指标检测结果显示,子痫前期孕妇的血尿素氮[(3.4±0.3)mmol/L]、肌酐[(63.4±2.9)μmol/L]以及C-反应蛋白[(7.3±1.0)mg/L]与正常孕妇[(2.9±0.3)mmol/L,(49.6±2.2)μmol/L,(2.9±0.9)mg/L]相比均显著升高(P＜0.05)。ELISA结果显示,子痫前期孕妇血清CXCL9[(269.6±19.7) pg/mL]和CXCL12水平[(110.4±5.3) pg/mL]与正常孕妇CXCL9[(212.5±9.6) pg/mL]和CXCL12[(83.6±3.3) pg/mL]相比显著升高(P＜0.05),CXCL10水平差异无统计学意义;Western blot结果也显示,子痫前期孕妇胎盘组织CXCL9和CXCL12水平与正常孕妇相比均显著上升(P＜0.05),而CXCL10水平差异无统计学意义。子痫前期孕妇分娩的新生儿血清CXCL9[(135.4±12.2 )pg/mL]和CXCL10水平[(82.9±6.2) pg/mL]与正常孕妇分娩的新生儿[(208.3±1.4)pg/mL,(139.5±2.1) pg/mL]相比均显著降低(P＜0.05),但CXCL12水平[(108.2±2.8) pg/mL]与正常孕妇的新生儿[(85.4±2.3) pg/mL]相比显著升高(P＜0.05)。此外,子痫前期孕妇血清CXCL9与肾功能指标BUN、Cr呈正相关性,CXCL12与FGR呈正相关性。结论孕妇子痫前期所表现的肾功能损伤,与趋化因子CXCL9有关;而胎儿宫内生长受限的发生则与趋化因子CXCL12有关。

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关键词 ：子痫前期, 孕妇, 新生儿, 趋化因子

收稿日期: 2020-05-20

ZTFLH:

R71

基金资助:国家自然科学基金面上项目(81173290)

通讯作者: 贺丰杰(hefengjie2013@163.com)

引用本文:

王芙蓉, 严谨, 贺丰杰. 子痫前期患者及其胎儿血清、胎盘组织中趋化因子的表达水平变化及意义[J]. 中国生育健康杂志, 2022, 33(2): 151-155.

链接本文:

http://cjrh.bjmu.edu.cn/CN/ 或 http://cjrh.bjmu.edu.cn/CN/Y2022/V33/I2/151

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