Abstract：Objective To explore the effects of mechanical stress on proliferation and collagen metabolism of female parauterine ligament fibroblasts. Methods Five patients aged 43~49 years who underwent total hysterectomy for benign tumors in the gynecology department of Shanghai First Maternity and Infant Hospital were selected during February 2020 to May 2020.All patients had no pelvic floor dysfunction disease and were not postmenopausal.The primary fibroblasts of human uterosacral ligament were obtained and identified by enzyme digestion.Parauterine fibroblasts were subjected to cyclic tensile stress loading at different times(12 and 24 h) and different sizes(6%, 12% and 18%).The proliferation ability of fibroblasts and the mRNA expression changes of Collagen metabolism-related genes MMP-2, TIMP-1, Collagen I, Collagen III and LOXL1 were detected. The non stressed cell group was used as the control group in the comparision analysis. Results Compared with the control group, tensile stress in 6%, 12% and 18% group could inhibit the proliferation of fibroblasts respectively, and the effect of inhibition increased with the increase of stress(from 6% to12%) and the extension of treatment time(from 12h to 24 h) in a certain range.Compared with the control group, the RNA levels of MMP-2, TIMP-1, Collagen I, Collagen III and LOXL1 in fibroblasts were slightly up-regulated at group of tensile stress(6%), but there was no significant difference(P> 0.05).The RNA levels of MMP-2, TIMP-1, Collagen I, Collagen III and LOXL1 in fibroblasts were down-regulated at group of tensile stress(12% and 18%).The mRNA expressions of MMP-2, TIMP-1, Collagen I, Collagen III and LOXL1 were decreased with increasing force(from 12% to18%), and the mRNA expressions of LOXL1 were decreased significantly(P< 0.05); The mRNA expressions of MMP-2, Collagen I and TIMP-1 decreased significantly with the extension of treatment time(from 12h to24 h)(P< 0.05), while there was no significant difference in CollagenⅢ and LOXL1 mRNA expression(P< 0.05).Among the genes, the maximum downregulation of the expression levels of mRNA levels of Collagen III and MMP-2 were 21.1% and 24.8%, while the mRNA levels of TIMP-1, Collagen I and LOXL1 were 43.6%, 40.1% and 47.9%, respectively. Conclusion Mild tensile stress in a relatively short duration of seems to promote the proliferation of uterosacral ligament fibroblasts and up-regulate the expression of collagens related genes, however, further experimental verification is still needed.The proliferation of fibroblasts and the expression of collagen-related genes were inhibited by higher or longer tensile stress, but the downregulation of each gene was inconsistent.
胡烨, 张永莉, 杜贵强. 机械应力对人子宫旁韧带成纤维细胞增殖及胶原代谢的影响[J]. 中国生育健康杂志, 2023, 34(4): 307-312.
HU Ye, ZHANG Yongli, DU Guiqiang. Effects of mechanical stress on proliferation and collagen metabolism of female parauterine ligament fibroblasts. Chinese Journal of Reproductive Health, 2023, 34(4): 307-312.
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