Abstract：Objective To investigate the expression of Thymidylate Synthetase (TYMS) gene in the epithelial ovarian cancer and explore its correlated genes of TYMS, potential biological functions plus pathways by bioinformatics methods. Methods The expression level of TYMS in the epithelial ovarian cancer was analyzed based on the data from Oncomine databases. Then cBioPortal database was used to find its relevant genes, STRING database is used to construct PPI network, Visualization and Integrated Discovery (DAVID) is used for Gene Ontology (GO) function annotation, and Kyoto Encyclopedia of Genes and Genomes (KEGG) is used for pathways analysis about TYMS and relevant genes. Results Compared with normal ovarian tissues, TYMS gene was highly expressed in the ovarian cancer (P< 0.05). Enrichment analysis showed that the high expression of TYMS was related to the regulation of mitotic G1/S phase transition, cell cycle, pyrimidine metabolism, DNA replication, and base excision repair pathways. The upstream regulatory gene E2F3 of TYMS is a key factor in the regulation of proliferation in the G1/S phase. Conclusion The expression of TYMS in the epithelial ovarian cancer was significantly increased, and its biological functions and pathways are mainly related to cell cycle, DNA synthesis and repair. E2F3 may regulate cell cycle progression by binding to TYMS.
苏欣欣, 李晓阳, 邹丹, 杨丽华. 胸苷酸合成酶在上皮性卵巢癌中表达的相关生物信息学分析[J]. 中国生育健康杂志, 2023, 34(4): 313-318.
SU Xinxin, LI Xiaoyang, ZOU Dan, YANG Lihua. Bioinformatics analysis for the expression of TYMS in the epithelial ovarian cancer. Chinese Journal of Reproductive Health, 2023, 34(4): 313-318.
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