Effects of different sources of protein intervention on cerebellum metabolome of fetal and offspring rat
HONG Yuting, YANG Chen, ZHONG Jinjing, HOU Yanmei, XIE Kui, WANG Linlin
Institute of Reproductive and Child Health / National Health Commission Key Laboratory of Reproductive Health; Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing 100191, China
Abstract：Objective This study intends to explore the effects of different protein sources on the metabolome of the cerebellum of fetal and offspring rats by feeding different kinds of feed that contains different proportions of milk protein concentrate and soy protein isolate. Methods SD pregnant rats were intervened with feeds of different proportions of milk protein concentrate and soy protein isolate and divided into 3 groups, named S1(50% concentrated milk protein + 50% soybean protein isolate, n=24), S2(25% concentrated milk protein + 75% soybean protein isolate, n=24), and S3(100% soybean protein isolate, n=24). The intervention continued from gestational day 0.5 to gestational day 18.5 or postnatal day 21. On the gestational day 19.5 or postnatal day 21, the cerebellum of fetal and offspring rat was collected after fasting, and non-targeted metabolomics were detected by ultra-high performance liquid chromatography-mass spectrometry. Principal component analysis was used to observe the differences in metabolic profiles of samples between different groups; partial least squares discriminant analysis and single-factor statistical methods were combined to screen differential metabolites between groups, and further screen differential metabolites shared by different groups. Trend analysis of variance was performed on the levels of the substances in different groups. Results Principal component analysis showed that the metabolic profiles of the cerebellum of the fetal rat in S1 and S3 were distinct, and the metabolic profiles of the cerebellum of offspring rat in the S1 and S2, S1 and S3 were distinct. After comprehensive partial least squares discriminant analysis and single factor statistical method to screen differential metabolites, 11 common endogenous differential metabolites were found in the cerebellum of offspring rat between different groups. The levels of six substances increased linearly in S1-S3, including N-methylhistidine, 4-aminovaleric acid betaine, 1-methylnicotinamide, guanine, cytidine 5'-monophosphate and 1-methyladenosine. Conclusions Intervention of different proportions of milk protein concentrate and soy protein isolate could affect the metabolome of fetal and offspring cerebellum.
洪昱廷, 杨晨, 钟瑾璟, 侯艳梅, 谢奎, 王琳琳. 不同比例浓缩牛乳蛋白及大豆分离蛋白干预对胎鼠及仔鼠小脑代谢组的影响[J]. 中国生育健康杂志, 2023, 34(5): 458-465.
HONG Yuting, YANG Chen, ZHONG Jinjing, HOU Yanmei, XIE Kui, WANG Linlin. Effects of different sources of protein intervention on cerebellum metabolome of fetal and offspring rat. Chinese Journal of Reproductive Health, 2023, 34(5): 458-465.
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