血清miRNA-146a在多囊卵巢综合征患者中的表达水平及临床意义

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摘要目的探究miRNA-146a在多囊卵巢综合征(PCOS)患者中的临床诊疗意义。方法选择在本院妇产科检查的PCOS患者50例作为观察组,所有患者均符合鹿特丹PCOS诊断标准;另外,采用随机数字表法选取50例经诊断无雄激素过多或排卵功能异常的健康女性作为对照组。所有入组的患者均检测睾酮、催乳素、黄体生成素、卵泡刺激素、雌激素、葡萄糖和胰岛素生化指标。qRT-PCR检测血清miRNA-146a的表达水平。结果观察组患者睾酮、黄体生成素、雌激素、葡萄糖、胰岛素和催乳素的水平分别为(0.8±0.2) ng/ml、(9.8±3.1) U/L、(75.5±16.4)pg/ml、(5.9±1.2) mmol/ml、(17.4±4.5) μU/ml和(33.7±10.8) ng/ml,均显著高于对照组的(0.4±0.1) ng/ml、(6.9±2.0) U/L、(32.4±9.8) pg/ml、(4.1±0.9)mmol/ml、(6.1±1.2)μU/ml和(10.6±3.2)ng/ml;同时,观察组卵泡刺激素水平(4.1±2.3) U/L明显低于对照组的(7.7±3.4) U/L;观察组患者miRNA-146a血清表达水平为(2.53±0.78),明显高于对照组的(0.59±0.30);上述指标比较,两组差异均有统计学意义;血清中miRNA-146a表达水平和睾酮浓度呈显著的负相关性(R²=0.798;P<0.001)。结论 PCOS患者血清miRNA-146a表达水平升高,且其与睾酮呈负相关的作用,可作为一种快速、方便和有效诊断PCOS疾病的指标之一,在PCOS患者的疾病发展过程中起到重要的作用。

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关键词 ： miRNA-146a, 多囊卵巢综合征, 睾酮

收稿日期: 2016-05-16

引用本文:

方恋. 血清miRNA-146a在多囊卵巢综合征患者中的表达水平及临床意义[J]. 中国生育健康杂志, 2016, 27(6): 568-570.

链接本文:

http://cjrh.bjmu.edu.cn/CN/ 或 http://cjrh.bjmu.edu.cn/CN/Y2016/V27/I6/568

1 黄丽红,俞超芹,张丹英.多囊卵巢综合征高雄激素血症及慢性炎症的相关性研究进展.生殖与避孕,2014,34:936-939.
2 Hosseinpanah F,Barzin M,Keihani S,et al.Metabolic aspects of different phenotypes of polycystic ovary syndrome:Iranian PCOS Prevalence Study.Clin Endocrinol,2014,81:93-99.
3 彭小莲,韩劲松.多囊卵巢综合征与代谢综合征.中国微创外科杂志,2014,20:77-80.
4 张庆勇,李乾元,罗春华.肿瘤标志物外周血microRNA的研究进展.细胞与分子免疫学杂志,2015,31:419-421.
5 Khan GH,Galazis N,Docheva N,et al.Overlap of proteomics biomarkers between women with pre-eclampsia and PCOS:a systematic review and biomarker database integration.Hum Reprod,2015,30:133-148.
6 Tsai YH,Wang TW,Wei HJ,et al.Dietary intake,glucose metabolism and sex hormones in women with polycystic ovary syndrome (PCOS) compared with women with non-PCOS-related infertility.Br J Nutr,2014,109:2190-2198.
7 Fauser B,Chang J,Azziz R,et al.The Rotterdam ESHRE/ASRM–Sponsored PCOS consensus workshop group.Revised 2003 consensus on diagnostic criteria and long–term health risks related to polycystic ovary syndrome(PCOS).Hum Reprod,2004,19:41-47.
8 Xu X,Yang X,Ru G,et al.miR-146a gene polymorphism rs2910164 and the risk of digestive tumors:A meta-analysis of 21 case-control studies.Oncol Rep,2014,31:472-479.
9 Wang L,Chopp M,Szalad A,et al.The role of miR-146a in dorsal root ganglia neurons of experimental diabetic peripheral neuropathy.Neuroscience,2014,259:155-163.
10 Anna Z,Stefan K,Ignat D,et al.Plasma microRNA profiling reveals loss of endothelial miR-126 and other microRNAs in type 2 diabetes.Circ Res,2010,107:810-817.
11 Baley J,Li J.Micrornas and ovarian function.J Ovarian Res,2012,5:8.
12 Long W,Zhao C,Ji CB,et al.Characterization of serum microRNAs profile of PCOS and identification of novel non-invasive biomarkers.Cell Physiol Biochem,2014,33:1304-1315.
13 Murri M,Insenser M,Fernández-Durán E,et al.Effects of polycystic ovary syndrome ( PCOS) ,sex hormones,and obesity on circulating miRNA-21,miRNA-27b,miRNA-103,and miRNA-155 expression.J Clin Endocrinol Metab,2013,98:1835-1844.
14 Chen H,Lan HY,Roukos DH,et al.Application of microRNAs in diabetes mellitus.J Endocrinol,2014,222:1-10.
15 Cochrane DR,Spoelstra NS,Richer JK.The role of miRNAs in progesterone action.Mol Cell Endocrinol,2012,357:50-59.